- 6-His (See other available formats)
- Regulatory Status
- Other Names
- polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).Product Details
- Verified Reactivity
- 6xHis Tag
- Antibody Type
- Host Species
- Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/mL (1:200-1:1,000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
(PubMed link indicates BioLegend citation)
- Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB)
- Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed
- Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB)
- Bartsch D, et al. 1998. Cell. 95:211. (ICC)
- Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
- Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed
- Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
- Product Citations
AB_2750116 (BioLegend Cat. No. 906115)
AB_2750117 (BioLegend Cat. No. 906116)
- Biology Area
- Cell Biology
- Gene ID
- View information about 6-His on UniProt.org
Related Pages & Pathways
Other FormatsView All 6-His Reagents Request Custom Conjugation
|Anti-6-His Epitope Tag Affinity Matrix||6-His||IP,Purification|
|Biotin anti-6-His Epitope Tag||6-His||WB,ICC,IP|
|FITC anti-6-His Epitope Tag||6-His||ICC|
|Purified anti-6-His Epitope Tag||6-His||WB,ICC,IP|
|Direct-Blot™ HRP anti-6-His Epitope Tag||6-His||WB|
|Alexa Fluor® 647 anti-6-His Epitope Tag||6-His||ICC|
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