- Clone
- RTK4530 (See other available formats)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
- publications
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 400661 | 100 µg | 128€ | ||||
The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Trinitrophenol + KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- This antibody was purified by affinity chromatography, and conjugated with Alexa Fluor®: 584 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-F - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
-
Application References
(PubMed link indicates BioLegend citation) -
- Cervantes-Barragan L, et al. 2007. Blood 109:1131.
- Zeiser R, et al. 2007. Blood 109:2225.
- Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
- Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
- Yi H, et al. 2009. Blood 113:5819. PubMed
- Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
- Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
- Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Antigen Details
- Gene ID
- NA
- UniProt
- View information about IgG2b kappa on UniProt.org
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