- QE5 (See other available formats)
- Regulatory Status
- Other Names
- HNUP153, N153, Nuclear Pore Complex Protein NUP153, Nucleoporin Nup153, 153 kD nucleoporin, nuclear pore complex protein hnup153
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
Nucleoporin 153 (Nup153) is a component of the nuclear pore complex, and is required for the anchoring of the nuclear pore complex to the nuclear pore membrane. Nup153 has a critical role in the nuclear export of proteins and RNA, and is involved in the retention of unspliced mRNAs in the nucleus.Product Details
- Antibody Type
- Host Species
- The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Application Notes
This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP).
*Predicted MW = 250 kD
This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
(PubMed link indicates BioLegend citation)
- Pare GC, et al. 2005. Exp Cell Res. 303:388.
- N Pante, et al. 1994. J Cell Biol. 126:603.
- Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
- Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
- Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
- Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
- Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
- Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
- Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
AB_2572062 (BioLegend Cat. No. 906204)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.