Alexa Fluor® 594 anti-NUP153 Antibody

Pricing & Availability
Clone
QE5 (See other available formats)
Regulatory Status
RUO
Other Names
HNUP153, N153, Nuclear Pore Complex Protein NUP153, Nucleoporin Nup153, 153 kD nucleoporin, nuclear pore complex protein hnup153
Isotype
Mouse IgG1
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Product Citations
publications
QE5_A594_NUP153_Antibody_IF_021618
HeLa cells were fixed with 100% ice-cold methanol for 15 minutes at -20°C followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 5 µg/ml Alexa Fluor® 594 NUP153 antibody (clone QE5, red). Nuclei were counterstained with DAPI (blue).
  • QE5_A594_NUP153_Antibody_IF_021618
    HeLa cells were fixed with 100% ice-cold methanol for 15 minutes at -20°C followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 5 µg/ml Alexa Fluor® 594 NUP153 antibody (clone QE5, red). Nuclei were counterstained with DAPI (blue).
See Alexa Fluor® 594 spectral data
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906204 100 µg 276,00€
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Description

Nucleoporin 153 (Nup153) is a component of the nuclear pore complex, and is required for the anchoring of the nuclear pore complex to the nuclear pore membrane. Nup153 has a critical role in the nuclear export of proteins and RNA, and is involved in the retention of unspliced mRNAs in the nucleus.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Eukaryote
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP).

*Predicted MW = 250 kD

This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.

Application References

(PubMed link indicates BioLegend citation)
  1. Pare GC, et al. 2005. Exp Cell Res. 303:388.
  2. N Pante, et al. 1994. J Cell Biol. 126:603.
  3. Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
  4. Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
  5. Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
  6. Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
  7. Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
  8. Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
  9. Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
RRID
AB_2572062 (BioLegend Cat. No. 906204)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Gene ID
9972 View all products for this Gene ID
UniProt
View information about NUP153 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/20/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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