Alexa Fluor® 594 anti-Nestin Antibody

Pricing & Availability
Clone
Rat-401 (See other available formats)
Other Names
Nestin, NES
Isotype
Mouse IgG1, κ
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Rat-401_A594_Nestin_Antibody_IHC-P_122617
IHC staining of Alexa Fluor® 594 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded embryonic day 15 mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40x objective. Scale bar: 20 µm.
  • Rat-401_A594_Nestin_Antibody_IHC-P_122617
    IHC staining of Alexa Fluor® 594 anti-Nestin antibody (clone Rat-401) on formalin-fixed paraffin-embedded embryonic day 15 mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40x objective. Scale bar: 20 µm.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
655105 25 µg 108€
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655106 100 µg 244€
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Description

Nestin is a class IV intermediate filament (IF) protein of approximately 240 kD. Nestin interacts with other IF proteins including vimentin, desmin and internexin to form heterodimers and mixed polymers. Nestin is expressed in a variety of neural stem and progenitor cells, and in early stages of development in the central nervous system (CNS). Upon differentiation, and during neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments such as neurofilaments and glial fibrillary acidic protein (GFAP). Nestin distribution and expression pattern in proliferating cells suggests that it participates in structural organization of the cell by regulating the assembly and disassembly of intermediate filaments. The function of nestin itself is highly regulated by phosphorylation. Nestin expression is induced in adults under pathological conditions such as glial scar formation after CNS injury. Antibodies against nestin can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against Homogenized Sprague-Dawley rat spinal cord.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The reactivity of Alexa Fluor® 647 anti-Nestin antibody was validated in FFPE embryonic mouse tissue.

Additional reported applications (for the relevant formats) include: immunofluorescence, immunohistochemistry, and immunoprecipitation1-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hockfield S, et al. 1985. J. Neurosci. 5:3310. (IP)
  2. Friedman B, et al. 1990. J. Comp. Neurol. 295:43. (IP)
  3. Brook GA, et al. 1994. Exp. Neurol. 126:31. (IP)
  4. Lendahl U, et al. 1990. Cell 60:585. (IP)
RRID
AB_2721390 (BioLegend Cat. No. 655105)
AB_2721391 (BioLegend Cat. No. 655106)

Antigen Details

Structure
Mouse nestin is 1864 amino acid protein with a predicted molecular weight of 207 kD. The observed molecular weight is larger than 250 kD.
Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus and cytosol.

Function
Nestin gene encodes a member of the intermediate filament, and it is required for survival and renewal of neural progenitor cells.
Interaction
Nestin forms homodimers and homotetramers in vitro. It also interacts with other intermediate filament proteins such as vimentin and alpha-internexin.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Xie L. et al. 2015. Stem Cells Int. (2015):762098.
2. Zhao Z, et al. 2014. Breast Cancer Res. 16(4):408.
3. Narita K, et al. 2014. Int. J. Oncol. 44(4):1118
4. Klein D, et al. 2014. Front Oncol. 4:169
5. Jin X, et al. 2013. Biochem. Biophys. Res. Commun. 433(4):496
6. Su HT, et al. 2013. Mol. Cancer Res. 11(7):768.

Gene ID
18008 View all products for this Gene ID 25491 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org

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Go To Top Version: 1    Revision Date: 12/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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