Alexa Fluor® 594 anti-human/mouse SSEA-3 Antibody

Pricing & Availability
Clone
MC-631 (See other available formats)
Other Names
Stage-Specific Embryonic Antigen-3
Isotype
Rat IgM, κ
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Product Citations
publications
MC-631_A594_SSEA3_Antibody_ICC_050719
Human stem cell line NCCIT was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of anti-human SSEA3 (clone MC-631) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
  • MC-631_A594_SSEA3_Antibody_ICC_050719
    Human stem cell line NCCIT was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of anti-human SSEA3 (clone MC-631) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
330316 100 µg 276€
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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
4-8 cell stage mouse embryos
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. EMBO 2:2355. (IF)
  2. Kaufmann B, et al. 2005. Virology 332:189. (ELISA)
RRID
AB_2563228 (BioLegend Cat. No. 330316)

Antigen Details

Distribution

Embryonic stemcells

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells
Gene ID
93961 View all products for this Gene ID
UniProt
View information about SSEA-3 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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