- C8/144B (See other available formats)
- Regulatory Status
- Other Names
- T8, Leu2
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Host Species
- A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Application Notes
Additional reported applications (for the relevant formats) include activation3, flow cytometry4, immunohistochemical staining of frozen tissue sections2, and Western blotting5.
(PubMed link indicates BioLegend citation)
- McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88. (IHC-P)
- Petukhova L, et al. 2010. Nature 466:113. (IHC-F)
- Clement M, et al. 2011. J. Immunol. 187:654. (Activ)
- MarTchal R, et al. 2010. BMC Cancer 10:340. (FC)
- Pontiggia L, et al. 2008. J. Invest. Dermatol. 129:480. (WB)
AB_2650658 (BioLegend Cat. No. 372904)
- Ig superfamily, homodimer or heterodimer with CD8β.
Majority of thymocytes, T cell subset, NK cells.
- MHC class I co-receptor, thymic differentiation, T-cell activation.
- MHC class I molecules.
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88.
- Gene ID
- 100387674 View all products for this Gene ID
- View information about CD8a on UniProt.org
|Purified anti-human CD8a||C8/144B||IHC-P,Activ,FC,IHC-F,WB|
|Alexa Fluor® 594 anti-human CD8a||C8/144B||IHC-P|
|Alexa Fluor® 647 anti-human CD8a||C8/144B||IHC-P,ICFC,3D IHC|
|Biotin anti-human CD8a||C8/144B||IHC-P|
|Spark YG™ 570 anti-human CD8a||C8/144B||IHC-P|
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.