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Alexa Fluor® 488 anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
1_FA-11_AF488_052710
Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 488
  • 1_FA-11_AF488_052710
    Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 488
  • 2_FA-11_A488_CD68_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD68 Antibody (clone FA-11) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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137011 25 µg 118€
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137012 100 µg 278€
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Barbour T, et al. 2016. Kidney Int. 89: 823-832. PubMed
  2. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  3. Zhou Y, et al. 2019. Differentiation. 109:16:00. PubMed
  4. Wang LH, et al. 2021. Sci Adv. 7: . PubMed
  5. Paterson N, et al. 2022. Elife. 11:. PubMed
  6. García L, et al. 2015. Exp Biol Med. 240: 904 - 916. PubMed
  7. DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
  8. Lv F, et al. 2022. iScience. 25:103639. PubMed
  9. Vegas A, et al. 2016. Nat Biotechnol. 10.1038/nbt.3462. PubMed
  10. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  11. Kawasaki N, et al. 2013. Proc Natl Acad Sci U S A. 110:7826. PubMed
  12. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  13. DeDreu J, et al. 2022. FASEB J. 36:e21995. PubMed
  14. Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed
RRID
AB_2074847 (BioLegend Cat. No. 137011)
AB_2074846 (BioLegend Cat. No. 137012)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org
Go To Top Version: 4    Revision Date: 09/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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