Product: FITC anti-human CD154 |
Catalog No.: 310804 |
Farag Ahmed, Msc, BMBCh Hannover Medical School (MHH) |
Nicely Staining Antibody (FITC anti-human CD154) | |||
Overall: | Product Quality: | Ease of Use: | |
Bright staining. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Human PBMC |
Brief Protocol: | Stimulation with (super)antigen and anti-CD28 over night. For stabilizing Cd154, anti-CD40 was used. Antibody staining: 30min on ice. Gated on lymphocytes, singlets and CD3+. |
Results Summary: | Bright population of activated T cells. |
Additional Notes: |
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