Product: FITC anti-mouse CD19 |
Catalog No.: 115506 |
J Lykken, Dr. Duke University |
Clearly labels CD19+ mouse splenocytes | |||
Overall: | Product Quality: | Ease of Use: | |
Clearly and consistently labels CD19+ mouse splenocytes. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Mouse Splenocytes |
Brief Protocol: | Isolate splenocytes from mice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Block Fc receptors and stain for viability in PBS for 20 minutes on ice. Wash cells once in FACS buffer (PBS plus 1% BSA or serum). Stain with isotype control or CD19 FITC antibody (1.6 ug/mL in FACS buffer, 1:250 dilution, 100 uL per million cells) for 20 minutes on ice. Wash cells once in FACS buffer. Resuspend cells in 100-250 uL FACS buffer of 1.5% paraformaldehyde. |
Results Summary: | Clearly and consistently labels CD19+ mouse splenocytes. |
Additional Notes: |
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