Product: Brilliant Violet 510™ anti-mouse/human CD11b |
Catalog No.: 101245 |
Anushka George, RA KSU |
Brilliant Violet™ Antibodies (anti-mouse/human CD11b) | |||
Overall: | Product Quality: | Ease of Use: | |
K9 immunophenotyping using CD11b BV510 for cell surface staining. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Canine whole blood |
Brief Protocol: | Stain whole blood with CD11b BV510. Incubate in dark for 30 mins. Lyse cells using multi-species lysis buffer. Wash with PBS and read on cytometer. |
Results Summary: | CD14+ staining seen on monocytes and granulocytes. Also shows cross reactivity with K9 species. |
Additional Notes: |
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Technical Service Notes |
BioLegend does not regularly test our antibodies against canine blood. In addition, not all antigens stain well in whole blood (compared to staining of PBMCs, for example). |
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