Product: Biotin anti-mouse Podoplanin

Catalog No.: 127403

Ela Elyada, Dr Cold Spring Harbor Laboratory

Biotin anti-mouse Podoplanin Works in IHC
Overall:	Product Quality:	Ease of Use:
This Ab works very good in FFPE staining IHC.

Experimental Design

Application:	IHC
Cells used:	pancreatic tumor tissue
Brief Protocol:	Standard deparaffinization and rehydration, antigen retrieval in 10mM Citrate buffer pH6 in a pressure cooker. 3% H2O2 blocking for 20 mins, blocking with 2.5% Horse serum for 30 mins, Avidin/Biotin blocking system (15 mins Avidin solution followed by 15 mins Biotin solution), primary Ab 1:500 O/N @ 4 C, washes and incubation with SA-HRP ready to use solution, wash and develop with DAB, Hematoxylyn, cleaning and mount.
Results Summary:	Ab stains stromal cells very specific and clean. Do not stain epithelial cells, as expected.
Additional Notes:

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Publication Status This data has not been published.

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