Product: ELISA MAX™ Standard Set Mouse IL-6 |
Catalog No.: 431301 |
Goutham Pattabiraman, Postdoctoral Fellow University of Connecticut Health Center |
Mouse IL-6 ELISA | |||
Overall: | Product Quality: | Ease of Use: | |
ELISA for cell culture supernatants from BMDMs |
Experimental Design |
Application: | ELISA |
Cells used: | Bone Marrow derived macropghages |
Brief Protocol: | Dilute capture antibody to the coating buffer as prescribed in the sheets and add it to the plate. After overnight incubation, wash, add block the plate. After 1 hour wash, add standards and samples. After 2 hours, wash, add detection antibody. After 1 hour, wash, add the enzyme avidin HRP. After 30 minutes, wash, add TMB solution, and after 10-20 minutes, add stop the reaction using an acid. Read the plate. |
Results Summary: | Good range of detection |
Additional Notes: |
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