Product: APC anti-mouse CD279 (PD-1) |
Catalog No.: 135210 |
Andreia Lino, PhD Deutsches Rheuma-Forschungszentrum, a Leibniz Institute |
Anti-mouse CD279 (PD-1) APC for Flow Cytometry | |||
Overall: | Product Quality: | Ease of Use: | |
APC anti-mouse CD279 (PD-1) is a good option. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | Resuspend in PBS/BSA and count the cells. Load up to 5x106 cells onto a V-Bottom 96-well-plate. Spin down, resuspend in 50uL of PBS/BSA containing αFcγR (2.4G2) 30 µg/ml and incubate 15min at 4°C. Spin down and resuspend in 50 µl antibody staining solution containing anti-PD-1 APC (1:100). Incubate 15min at 4°C in the dark. Wash twice with PBS/BSA, resuspend and transfer to FACS tubes to read. Use PI to exclude the dead cells. |
Results Summary: | Some CD4 and fewer CD8 T cells express PD-1 in the spleen of naïve mice. |
Additional Notes: |
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