Product: Recombinant Human M-CSF (carrier-free) |
Catalog No.: 574802 |
Umesh Karandikar, Staff Scientist Baylor College of Medicine |
Works Great for Monocyte Derived Macrophages (Recombinant Human M-CSF) | |||
Overall: | Product Quality: | Ease of Use: | |
Carrier free MCSF efficiently differentiate PBMC derived monocytes into M0s. |
Experimental Design |
Application: | Bioassay |
Cells used: | Monocytes |
Brief Protocol: | 5 days of differentiation starting with 50ng/ml of MCSF for 48 hrs. Change media containing supplement with 10ng/ul of MCSF after 48hrs. Spike the media with additional 10ng/ml of MCSF. |
Results Summary: | Successful differentiation based on adherence and expression of surface markers by flow and IF. |
Additional Notes: |
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