Product: Human TruStain FcX™ (Fc Receptor Blocking Solution) |
Catalog No.: 422302 |
Jonathan Eby, Graduate Student Loyola University Chicago |
Fc Block and THP-1 Cells (Human TruStain FcX™) | |||
Overall: | Product Quality: | Ease of Use: | |
Increased antibody sensitivity and reduced background after Fc Blocking. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | THP-1 cells |
Brief Protocol: | Cells spun down directly from incubator. Cells were incubated 10 minutes in PBS + 2% human serum in addition to the Human TruStain FcX block. Following incubation, primary antibodies were added and incubated at 4 C for 30 minutes, washed 2x, then incubated with secondary antibodies for 30 minutes at 4 C. Cells were washed 2x, then immediately ran on a cytometer. |
Results Summary: | Human TruStain FcX greatly reduced background staining seen typically from incubation with secondary antibody on this cell line. |
Additional Notes: |
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