Product: APC anti-mouse/human CD11b

Catalog No.: 101212

Leif Anderson, Graduate Student Researcher UC Davis

Quantifying Neutrophils in Bone Marrow (APC anti-mouse/human CD11b)
Overall:	Product Quality:	Ease of Use:
Number of neutrophils in femur were measured in C57BL/6 mice given a 6mm skin wound.

Experimental Design

Application:	Flow Cytometry
Cells used:	C57BL/6 Bone Marrow
Brief Protocol:	Isolate bone marrow cells to a concentration of 10E7 per ml in Cell Staining Buffer (Biolegend). Stain 100ul of cell suspension. Add leaf anti-CD16/32 (Biolegend) to 1 ug/ml for 10 minutes. Add 1ul of APC anti-mouse CD11b (and 1ul PE/Cyanine7 anti-mouse Ly-6G) to cell suspension. Stain for 20' at RT in darkness. Wash with 1ml cell staining buffer, spin at 400xG for 5 minutes. Resuspend in 500 ul cell staining buffer. Assess on Attune NxT (Record 200 ul at 200 ul/min)
Results Summary:	After wounding, the number of neutrophils in bone marrow drops and then steadily rises over 5 days.
Additional Notes:	Great separation. This antibody is good for detecting neutrophils. I have also used this antibody successfully in expanding human CD34 cells as a marker for myelopoiesis.

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Publication Status This data has not been published.

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