Product: FITC anti-mouse/rat CD29 |
Catalog No.: 102205 |
Adam Guess, SR Research Associate The Research Institute at Nationwide Children's Hospital |
Staining Mouse Bone Marrow with anti-mouse CD29 FITC | |||
Overall: | Product Quality: | Ease of Use: | |
Anti-CD29 FITC works well on mouse bone marrow. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse bone marrow |
Brief Protocol: | Bone marrow was flushed from femurs and tibiae with PBS/2%FBS through a 27 gauge needle and a 40uM strainer. Cells were washed, counted, and stained for 15 minutes at room temperature with the recommended dilution of anti-CD29 FITC. Cells were washed twice and analyzed on a BD LSR II. |
Results Summary: | CD29 staining was excellent (filled histogram), as compared to unstained cells (dotted line). |
Additional Notes: |
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