Product: Zombie NIR™ Fixable Viability Kit |
Catalog No.: 423105 |
Maria Carmela Speranza, Research Fellow DFCI |
Zombie NIR™ Fixable Viability Kit | |||
Overall: | Product Quality: | Ease of Use: | |
Great live/dead cell marker. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Spleenocytes, TILs |
Brief Protocol: | Dilute Zombie NIR 1:15 in FACS buffer. After cell extraction wash your sample 2x with washing buffer, stain for 30min RT with the already diluted 1:15 NIR 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol. |
Results Summary: | Splenocyte extracted from brain tumor-bearing mice. |
Additional Notes: |
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