Product: Brilliant Violet 605™ anti-mouse/human CD11b

Catalog No.: 101237

Mikhail Binnewies, Graduate Student UCSF

Anti-CD11b Brilliant Violet 605™ Staining Following Enzymatic Digestion
Overall:	Product Quality:	Ease of Use:
Anti-CD11b Brilliant Violet 605™ staining works well following collagenase I, IV and DNAse digestion in the lymph node.

Experimental Design

Application:	Flow cytometry
Cells used:	Enzymatically digested lymph node cells
Brief Protocol:	Inguinal lymph nodes were pierced and dissociated in 100 U/ml collagenase I, 400 U/ml collagenase IV and 200 ug/ml DNAse I for 30 minutes at 37C. Digested cells were filtered, washed with DPBS and stained with fixable Zombie NIR™. Cells were washed with PBS/2% FCS/2mM EDTA and stained with anti-CD11b Brilliant Violet 605™ (1:1000) and anti-Ly6C Brilliant Violet 711™ (1:2000). Plot shown below is after gating out B and T cells.
Results Summary:	Staining of anti-CD11b Brilliant Violet 605™ works very well following enzymatic digestion.
Additional Notes:	The staining was bright enough that the antibody could be used at a lower concentration.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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