Product: ELISA MAX™ Standard Set Mouse IL-1β |
Catalog No.: 432602 |
Midic Ivana, PhD University of Montenegro |
Mouse IL-1β ELISA MAX™ Standard | |||
Overall: | Product Quality: | Ease of Use: | |
Confirming the inflammatory cytokines produced from macrophages under experimental conditions. |
Experimental Design |
Application: | ELISA |
Cells used: | Macrophages |
Brief Protocol: | 1) Dilute capture antibody to the coating buffer as prescribed in the sheets and add it to the plate. 2) After overnight incubation wash + add block the plate. 3) After 1 hour wash + add standards and samples. 4) After 2 hours wash + add detection antibody. 5) After 1 hour wash + add the enzyme avidin HRP. 6) After 30 minutes wash + add TMB solution. 7) After 10-20 minutes stop the reaction using an acid. 8) Read the plate. |
Results Summary: | Confirming the inflammatory cytokines produced from macrophages under experimental conditions. |
Additional Notes: |
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