Product: PE anti-mouse/human CD11b |
Catalog No.: 101208 |
Ashley Milam, Postdoctoral Research Fellow Washington University in St. Louis |
Mouse anti-CD11b PE | |||
Overall: | Product Quality: | Ease of Use: | |
Purification of CD4+ splenocytes by gating out contaminating APCs. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Mouse splenocytes |
Brief Protocol: | RBC lysis of splenocytes; wash; 30 minute antibody stain in the dark on ice |
Results Summary: | Contaminating CD11b+ cells can be easily identified during purification sort. |
Additional Notes: |
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Technical Service Notes |
APCs likely indicates antigen presenting cells and not the fluor. |
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