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Best Practices
Staying up to date on the latest innovations in the flow cytometry field ensures your laboratory is implementing the best practices and techniques for your experiments.
Browse through our selection of resources and find a learning style that suits you: written protocols, step-by-step video protocols, informative blogs, webinars from academic and industry-leading experts, and literature that demonstrates complex pathways.
Protocols
Preparation is key for a successful flow cytometry experiment. Explore our fully detailed written protocols below or browse through our Video Library for step-by-step guided instructions on how to perform common flow cytometry assays.
Web Tools
Need help building your flow panel? We have over a dozen tools to guide you in choosing the right reagents. Learn about the spectral properties of fluorophores, find antibody selection tools, and get advice on troubleshooting and experimental setup.
View our flow cytometry web tools >
Videos
Watch our exclusive videos to gain insights from experts on new fluorophores, panel-building techniques, and additional experimental considerations.
Tech Insights - Biological Controls
Karen Morley, PhD, covers important factors to consider when setting up biological controls for your flow cytometry experiments, including positive, negative, and longitudinal controls.
Tech Insights - Buffers
Avi Perna reviews BioLegend's flow cytometry buffers and provides advice on which ones are ideal to prepare, fix, and intracellularly stain samples.
Tech Insights - Fixation
Ekaterina Zvezdova, PhD, explains how fixation can be helpful in flow cytometry. She also discusses important factors to consider when preparing samples for this process.
Tech Insights - Flow Cytometry Instrument Controls
Watch as Theresa Mendoza, MS, discusses setting up proper instrument controls for a flow cytometer, including compensation beads, single-color controls, and voltage settings.
Tech Insights: Flow Cytometry Gating & Staining Controls
Theresa Mendoza, MS, reviews important staining and gating controls for flow cytometer experiments. Learn about FMOs, isotype controls, and unstained cells.
Expanding Multiparameter Cell Analysis
Kenta Yamamoto, Product Manager for Cell Analysis, walks through the utility of new fluorophores for flow cytometry and multiomic solutions for single-cell analysis.
Blogs
Dive deeper into flow cytometry and gain insight on experimental setups and important concepts by reading through our blogs. Our tips are useful for all levels of flow cytometry users.
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