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Purified anti-Oligodendrocyte-myelin glycoprotein Antibody

Pricing & Availability
Clone
A16003A (See other available formats)
Regulatory Status
RUO
Other Names
Omg, Omgp
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A16003A_PURE_OMG_Antibody_1_WB_021317
  • A16003A_PURE_OMG_Antibody_1_WB_021317
  • A16003A_PURE_OMG_Antibody_2_WB_021317
    (A) Total cell lysates from SH-SY5Y and tissue lysates from mouse and rat brain (15 µg protein each), and (B) 50 ng recombinant mouse OMG protein, mouse brain (M. brain) and rat brain (R. brain) total protein (15 µg each) were treated with or without 100 µg PNGase F at 37°C overnight to cleave the N-linked glycans from the proteins. All proteins were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL of purified anti-OMG antibody (clone A16003A). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody was used as a loading control.
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694602 100 µg 276 CHF
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Description

Oligodendrocyte myelin glycoprotein, also known as OMG, is a glycosylphosphatidylinositol (GPI) anchored protein expressed by oligodendrocytes, neurons, and astrocytes. OMG, along with Nogo (Neurite outgrowth inhibitor) and MAG (myelin-associated glycoprotein), belongs to a group of molecules located in central nervous system (CNS) myelin protein fractions with axon outgrowth inhibitory activity that prevents axonal regeneration after CNS injury. The neurite outgrowth inhibitory activities of these inhibitory molecules are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR). OMG also associates with Paired immunoglobulin-like receptor B (PirB). OMG contributes to the interactive process required for myelination in the central nervous system. OMG is also involved in the regulation of synaptic plasticity and is implicated in the inhibition of cell proliferation. The highly conserved leucine-rich repeat domain is required for inhibitory effect on both axon outgrowth and cell proliferation. Human OMG cDNA encodes a 440 amino acid residue GPI-anchored protein with a 24 amino acid signal peptide, eight leucine-rich repeats followed by five serine/threonine-rich repeats, and 23 amino acid propeptide. OMG has eleven potential N-glycosylation sites.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse OMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody also detects a non-specific protein of approximately 145 kD in the mouse brain. Clone A16003A does not cross-react with human and rat (in-house tested).

RRID
AB_2650732 (BioLegend Cat. No. 694602)

Antigen Details

Structure
440 amino acids, and predicted molecular weight is 49 kD. Due to N-glycosylation (11 glycosylation sites), the observed molecular weight ranges from 90 kD to 120 kD.
Distribution
Cell membrane
Function
Cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system.
Interaction
RTN4R, Central nervous system (CNS) axons.
Ligand/Receptor
NgR1 and PirB
Biology Area
Cell Adhesion, Cell Biology
Antigen References

1. Habib AA, et al. 1998. Oncogene 12:1525-31.
2. Wang KC, et al. 2002. Nature 417:941-944.
3. Kottis V, et al. 2002. J. Neurochem. 82:1566-9.
4. Huang JK, et al. 2005. Science 310(5755):1813-7.
5. Atwal JK, et al. 2008. Science 322(5903):967-70.
6. Raiker SJ, et al. 2010. J. Neurosci. 30:12432-45.
7. Huang JY, et al. 2012. Brain Res. 1437:1-15.
8. Kempf A, et al. 2014. PLoS Biol. 12:e1001763.

Gene ID
18377 View all products for this Gene ID
UniProt
View information about Oligodendrocyte-myelin glycoprotein on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.13.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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