Purified anti-mouse CD285 (TLR5) Antibody

Pricing & Availability
Clone
ACT5 (See other available formats)
Other Names
Toll-like receptor 5, TIL3, SLEB1, MELIOS
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
ACT5_Purified_TLR5_Antibody_FC_061914.jpg
TLR5-transfected Ba/F3 cells were stained with purified TLR5 (clone ACT5) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • ACT5_Purified_TLR5_Antibody_FC_061914.jpg
    TLR5-transfected Ba/F3 cells were stained with purified TLR5 (clone ACT5) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
148102 100 µg 225 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
TLR5-transfected Ba/F3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Shibata T, et al. Int Immunol. 24:613. (IP, FC)
  2. Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
Product Citations
  1. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
RRID
AB_2563693 (BioLegend Cat. No. 148102)

Antigen Details

Structure
Type 1 transmembrane protein, 98 kD
Distribution

Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium

Interaction
Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor
Flagellin
Cell Type
Dendritic cells, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 
3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 
4. Kinnebrew M, et al. 2012. Immunity. 36:276. 
5. Sebastiani G, et al. 2000. Genomics. 64:230.

Gene ID
53791 View all products for this Gene ID
UniProt
View information about CD285 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.07.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account