HRP anti-Myelin CNPase Antibody

Pricing & Availability
Clone
SMI 91 (See other available formats)
Regulatory Status
RUO
Other Names
CNPase, Cnp-1, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SMI-91_HRP_Myelin_CNPase_Antibody_1_032318
Western blot of HRP anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2.5 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SMI-91_HRP_Myelin_CNPase_Antibody_1_032318
    Western blot of HRP anti-Myelin CNPase antibody (clone SMI 91). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2.5 µg/mL of primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SMI-91_HRP_Myelin_CNPase_Antibody_2_032318
    IHC staining of HRP anti-Myelin CNPase antibody (Clone SMI 91) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.E.I.R (Cat. No. 928502), the tissue was incubated with 1 µg/mL of the primary antibody for one hour at room temperature. DAB was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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836405 25 µg 105,00 CHF
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836406 100 µg 265,00 CHF
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Description

High CNPase expression is seen in myelin producing cells, including oligodendrocytes and Schwann cells. CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (CNS). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth. The enzyme isolated from the mammalian brain is primarily a mixed dimer of approximately 94 kD. The dimer consists of a varied proportion of CNP1 (46 kD) and CNP2 (48 kD) subunits in various species. Since the enzyme is a myelin-associated enzyme, it is of considerable interest in the study of diseases and disorders in which myelin is affected, such as multiple sclerosis, subacute sclerosing panencephalitis, acquired immunodeficiency with CNS involvement, and peripheral neuropathies. The combination of clone SMI 91 with clone SMI 94 and/or clone SMI 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 5.0 µg/mL. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1,2,4,8 (IHC), immunocytochemistry5,6 (ICC), and western blot (WB).3,7

Additional Product Notes

The reactivity of HRP anti-Myelin CNPase antibody was verified in FFPE rat brain tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Samanta J, et al. 2010. Stroke. 2:357-62. (IHC-F) 
  2. Werner HB, et al. 2007. J. Neurosci. 27:7717-7730. (IHC-P) PubMed
  3. McFerran B, et al. 1997. J Cell Sci. 110:2979-2985. (WB)
  4. Trolle C, et al. 2014. BMC Neurosci. 15:60. (IHC-F) PubMed
  5. Liyanage VR, et al. 2013. Mol. Autism 4:46. (ICC)
  6. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  7. Yu C, et al. 2008. Glia. 56:877. (WB)
  8. Johnson GC, et al. 2014. Pathol. 51:146. (IHC-P) PubMed
RRID
AB_2728548 (BioLegend Cat. No. 836405)
AB_2728549 (BioLegend Cat. No. 836406)

Antigen Details

Structure
Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.
Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus, mitochondria, cytosol, and plasma membrane.

Function
CNPase detects developing and adult myelin, and developing oligodendrocytes and Schwann cells. CNPase distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Interaction
Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.
Cell Type
Oligodendrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Enzymes and Regulators, Phospho-Proteins
Antigen References
  1. Han H, et al. 2013. Biofactors. 39(3):233.
  2. Raasakka A, et al. 2014. Neurosci Bull. 30(6):956.
  3. Kim JY, et al. 2003. J. Neurosci. 23(13):5561-5571.
  4. Sprinkle TJ, et al. 1989. Crit. Rev. Neurobiol. 4:235-301.
  5. Ding BS, et al. 2013. PLoS One 8:e62150.
  6. Ferletta M, et al. 2011. Int. J. Cancer 129:45.
Gene ID
1267 View all products for this Gene ID
UniProt
View information about Myelin CNPase on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06.05.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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