- Sterile PBS
- Anti-human CD3 Antibody: Clone UCHT1 (Ultra-LEAF™ format, Cat. No. 300437)
- Anti-human CD3 Antibody: Clone OKT3 (Ultra-LEAF™ format, Cat. No. 317325)
- Note: Soluble forms of Ultra-LEAF™ purified UCHT1 (1µg/ml) or Ultra-LEAF™ purified HIT3a (0.01 - 0.1µg/ml) may be used to activate T cells from PBMC cell populations.
- Anti-human CD3 Antibody: Clone HIT3a (Ultra-LEAF™ format, Cat. No. 300331)
- Cell culture medium (e.g., RPMI-1640 or IMDM supplemented with 10% FBS and 2mM L-glutamine)
- Sterile single-cell suspension of Ficoll-Hypaque-purified peripheral blood mononuclear cells, isolated T cells, or T cell subsets
- 96-well flat-bottom tissue culture plates with lids (e.g., Costar® Cat. No. 3596)