Ultra-LEAF™ Purified anti-mouse CXCL10 (IP-10) Antibody

Pricing & Availability
Clone
A16079E (See other available formats)
Regulatory Status
RUO
Other Names
Interferon gamma-inducible protein 10 (IP-10), Interferon gamma induced factor, gIP-10, IFI10, INP10, cytokine responsive gene-2 (CRG2)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A16079E_ULEAF_CXCL10_Antibody_030722
Recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectants. Ultra-LEAF™ purified anti-mouse CXCL10 (IP-10) (clone A16079E) (purple squares) neutralizes recombinant mouse CXCL10 (IP-10) induced chemotaxis at 8 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner, whereas GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.6 - 3 µg/mL
  • A16079E_ULEAF_CXCL10_Antibody_030722
    Recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectants. Ultra-LEAF™ purified anti-mouse CXCL10 (IP-10) (clone A16079E) (purple squares) neutralizes recombinant mouse CXCL10 (IP-10) induced chemotaxis at 8 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner, whereas GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 0.6 - 3 µg/mL
Cat # Size Price Quantity Check Availability Save
604453 100 µg 358 CHF
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604454 1 mg 935 CHF
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Description

CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11. CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many inflammatory diseases, including arthritis, type I diabetes, experimental autoimmune encepahlomyelitis, atherosclerosis, allograft rejection, and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL10 (IP-10)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant mouse CXCL10 (IP-10) (Cat. No. 573604) at 8 ng/mL on mouse Baf3- mCXCR3 transfectants. ND50 range: 0.6 - 3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922639 (BioLegend Cat. No. 604453)
AB_2922639 (BioLegend Cat. No. 604454)

Antigen Details

Structure
Chemokine
Distribution

Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, and T cells

Function
CXCL10 chemoattracts CD4, Th1, CD8, and NK and NKT cells. CXCL10 is induced by inflammatory cytokines. NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling ability.
Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, and fibroblasts
Ligand/Receptor
CXCR3
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Luster AD, et al. 1985. Nature 315:672-6.
  2. Vanguri P, et al. 1990. J. Biol. Chem. 265:15049-57.
  3. Loos T, et al. 2008. Blood 112:2648-56.
  4. Crawford MA, et al. 2009. Infect. Immun. 77:1664-78.
  5. Campanella GS, et al. 2010. PLoS One 5:e12700.
  6. Hoerning A. 2011. Eur. J. Immunol. 41:2291-302.
Gene ID
15945 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03.07.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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  • Ultra-LEAF™ Purified anti-mouse CXCL10 (IP-10)

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