Ultra-LEAF™ Purified anti-mouse CD210 (IL-10 R) Antibody

Pricing & Availability
Clone
1B1.3a (See other available formats)
Regulatory Status
RUO
Other Names
IL-10R, Interleukin-10 Receptor
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
1b1_3a
C57BL/6 mouse splenocytes stained with 1B1.3a PE
  • 1b1_3a
    C57BL/6 mouse splenocytes stained with 1B1.3a PE
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112714 100 mg 3075,00 CHF
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112712 25 mg 1280,00 CHF
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112713 50 mg 2140,00 CHF
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112709 100 µg 100,00 CHF
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112710 1 mg 135,00 CHF
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112711 5 mg 490,00 CHF
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Description

CD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble mIL-10R-FLAG-His
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block)
  2. Wang W, et al. 2004. FASEB J. 18:1043. (Block)
  3. Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block)
  4. Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block)
  5. Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)
Product Citations
  1. Gottschalk RA, et al. 2019. Elife. 8:e46836. PubMed
  2. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  3. Kuan Y, et al. 2013. PLoS One. 8:72422. PubMed
  4. Murdock B, et al. 2014. J Immunol. 193:4107. PubMed
RRID
AB_2832378 (BioLegend Cat. No. 112714)
AB_2616680 (BioLegend Cat. No. 112712)
AB_2832377 (BioLegend Cat. No. 112713)
AB_11147950 (BioLegend Cat. No. 112709)
AB_11149684 (BioLegend Cat. No. 112710)
AB_2616679 (BioLegend Cat. No. 112711)

Antigen Details

Structure
Class II cytokine receptor family, 90-110 kD
Distribution

Thymocytes, T and B cells, NK cells, monocytes, macrophages

Function
Inhibits cytokine production, costimulates B cell proliferation and differentiation
Ligand/Receptor
IL-10
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267.
2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053.
3. Niemand C, et al. 2003. J. Immunol. 170:3263.
4. Corinti S, et al. 2001. J. Immunol. 166:4312.

Gene ID
16154 View all products for this Gene ID
UniProt
View information about CD210 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 08.16.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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