Ultra-LEAF™ Purified anti-human CD49e Antibody

Pricing & Availability
NKI-SAM-1 (See other available formats)
Regulatory Status
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Mouse IgG2b, κ
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Product Citations
Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified NKI-SAM-1, followed by anti-mouse IgG FITC
  • NKI-Sam-1_LEAF_020808
    Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified NKI-SAM-1, followed by anti-mouse IgG FITC
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328013 100 µg 270 CHF
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328014 1 mg 743 CHF
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CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
U937 cells
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  2. Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
AB_2860857 (BioLegend Cat. No. 328013)
AB_2860858 (BioLegend Cat. No. 328014)

Antigen Details

Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)

Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells

Adhesion, regulation of cell survival and apoptosis
Fibronectin, neural adhesion molecule L1
Cell Type
Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.

Gene ID
3678 View all products for this Gene ID
View information about CD49e on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD49e Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD49e NKI-SAM-1 FC, IP, ELISA
FITC anti-human CD49e NKI-SAM-1 FC
PE anti-human CD49e NKI-SAM-1 FC
APC anti-human CD49e NKI-SAM-1 FC
Ultra-LEAF™ Purified anti-human CD49e NKI-SAM-1 FC, IP, ELISA, Block
Go To Top Version: 1    Revision Date: 03.24.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD49e

  • FITC anti-human CD49e

  • PE anti-human CD49e

  • APC anti-human CD49e

  • Ultra-LEAF™ Purified anti-human CD49e


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