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Ultra-LEAF™ Purified anti-human CD352 (NTB-A) Antibody

Pricing & Availability
Clone
NT-7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
SLAMF6, LY108, TCOM, SF2000, NTBA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NT-7_LEAF_082306
Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified NT-7, followed by anti-mouse IgG FITC
  • NT-7_LEAF_082306
    Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified NT-7, followed by anti-mouse IgG FITC
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317211 100 µg 270 CHF
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317212 1 mg 743 CHF
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Description

NTBA (NK-, T-, and B-cell Antigen) is a 60 kD type I transmembrane glycoprotein. It is a member of Ig superfamily belonging to CD2/CD150 subfamily, also known as SLAMF6 (SLAM family member 6), LY108, TCOM, or SF2000. This antigen is expressed on NK cells, T cells (upregulated upon activation), and B cells. NTBA is its own ligand. The homophilic interaction of NTBA is involved in the induction of NK cytotoxicity, CD28 independent T cell costimulation, triggering Th1 cytokines production, and regulation of B cell tolerance. It may play a role in the regulation of autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of NTB-A
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, Stim - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: stimulation of NTB-A in a redirected lysis assay and blocking of NTB-A function3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317211 & 317212). Co-stimulation of cells with CD3 antibodies and clone NT-7 has been shown to induce activation rather than blocking function of these cells3.

Application References

(PubMed link indicates BioLegend citation)
  1. Messmer B, et al. 2006. J. Immunol. 176:4646. (FC)
  2. Ward J, et al. 2007. Blood 110:1207. (FC) PubMed
  3. Uzana R, et al. 2012. J. Immunol. 188:632. (Block) PubMed
  4. Baglaenko Y, et al. 2017. J. Immunol. 198: 3949. (Stim)
RRID
AB_2860836 (BioLegend Cat. No. 317211)
AB_2860837 (BioLegend Cat. No. 317212)

Antigen Details

Structure
Type I transmembrane tyrosine-phosphorylated glycoprotein, CD2/CD150 subfamily, 60 kD
Distribution

NK cells, T cells, B cells

Function
CD28 independent T cell costimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance
Ligand/Receptor
NTBA
Cell Type
B cells, NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Bottino C, et al. 2001. J. Exp. Med. 194:235.
2. Valdez PA, et al. 2004. J. Bio. Chem. 279:18662.
3. Flaig RM, et al. 2004. J. Immunol. 172:6524.
4. Falco M, et al. 2004. Eur. J. Immunol. 34:1663.
5. Kumar KR, et al. 2006. Science 312:1665.

Gene ID
114836 View all products for this Gene ID
UniProt
View information about CD352 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03.24.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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