Ultra-LEAF™ Purified anti-human CD213a2 (IL13Rα2) Antibody

Pricing & Availability
Clone
47 (See other available formats)
Other Names
IL13BP, CT19
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
47_LEAF_CD213a2_Purified_Antibody_FC_091013
Human melanoma cell line A375 was stained with Ultra-LEAF™ Purified CD213a2 (clone 47) (filled histogram) or LEAF™ Purified mouse IgG1, κ isotype control (open black histogram) followed by anti-mouse IgG-FITC. The staining was completely blocked by pre-incubation of the cells with recombinant human IL-13 (open red histogram).
  • 47_LEAF_CD213a2_Purified_Antibody_FC_091013
    Human melanoma cell line A375 was stained with Ultra-LEAF™ Purified CD213a2 (clone 47) (filled histogram) or LEAF™ Purified mouse IgG1, κ isotype control (open black histogram) followed by anti-mouse IgG-FITC. The staining was completely blocked by pre-incubation of the cells with recombinant human IL-13 (open red histogram).
Cat # Size Price Quantity Avail. Save
360303 100 µg 285 CHF
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Description

CD213a2, also known as IL13Rα2, is a type I transmembrane protein and member of the hematopoietin family. IL13Rα2 is the high affinity receptor for IL-13, but it possibly acts as a decoy receptor to antagonize IL-13Rα1/IL-4Ra-mediated IL-13 biological functions. IL13Rα2 is expressed by fibroblasts, smooth muscle cells, keratinocytes, and activated B cells. However, its presence on the cell surface is highly regulated. CD213a2 is also expressed in various types of cancer which makes it an attractive therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-13R alpha 2 Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ELISA, IHC-F, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: ELISA1, immunohistochemistry staining of frozen tissue sections1, and blocking (competes with IL-13 for binding).

Application References

(PubMed link indicates BioLegend citation)
  1. Balyasnikova IV, et al. 2012. J. Biol. Chem. 287:30215. (ELISA, IHC, Block)
RRID
AB_2562511 (BioLegend Cat. No. 360303)

Antigen Details

Structure
Member of the hematopoietin family, type I transmembrane protein, contains 1 fibronectin type III domain
Distribution

Activated B cells, fibroblasts, smooth muscle cells, keratinocytes

Function
Possible decoy receptor, antagonist of CD213a1, internalization of IL-13
Ligand/Receptor
IL-13
Cell Type
B cells, Fibroblasts
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mitchell J, et al. 2010. Curr. Opin. Investig Drugs. 11:527.
2. Strober W, et al. 2009. Curr. Mol. Med. 9:740.
3. Tabata Y and Khurana Hershey GK. et al. 2007. Curr. Allergy Asthma Rep. 7:338.

Gene ID
3598 View all products for this Gene ID
UniProt
View information about CD213a2 on UniProt.org

Related FAQs

Does BioLegend test each ULEAF™ antibody by functional assay?

No, BioLegend does not test ULEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each ULEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 03.21.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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