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Ultra-LEAF™ Purified anti-human CD157 Antibody

Pricing & Availability
Clone
W21007F (See other available formats)
Regulatory Status
RUO
Other Names
BST1
Isotype
Rat IgG2a, λ
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Product Citations
publications
W21007F_ULEAF_CD157_Antibody_1_122022
Human peripheral blood mononuclear cells were stained with anti-human CD14 APC/Cyanine7 and purified anti-human CD157 (clone W21007F) (left) or purified rat IgG2a, λ isotype control (right) followed by anti-rat IgG APC and anti-human CD45 Pacific Blue™. Data shown was gated on CD45-positive lymphocytes+ monocytes.
  • W21007F_ULEAF_CD157_Antibody_1_122022
    Human peripheral blood mononuclear cells were stained with anti-human CD14 APC/Cyanine7 and purified anti-human CD157 (clone W21007F) (left) or purified rat IgG2a, λ isotype control (right) followed by anti-rat IgG APC and anti-human CD45 Pacific Blue™. Data shown was gated on CD45-positive lymphocytes+ monocytes.
  • W21007F_ULEAF_CD157_Antibody_2_121322
    The myelomonocytic cell line U937 was labeled with Calcein AM (Cat. No. 425201), washed and plated in 96 well plates with the indicated concentration of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) or rat IgG2a, λ isotype control. After 45 minutes, Calcein fluorescence was measured using a SpectraMax plate reader.
  • W21007F_ULEAF_CD157_Antibody_3_121322
    Human peripheral blood mononuclear cells were treated with the indicated concentration of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) or rat IgG2a, λ isotype control and plated on tissue culture plates coated with 50 µg/mL recombinant human FN-1 (carrier-free) (Cat. No. 775306). After 1 hour, non-adherent cells were removed by washing and adherent cells counted by brightfield microscopy. Data shown are fold changes normalized to isotype controls.
  • W21007F_ULEAF_CD157_Antibody_4_121322
    The myelomonocytic cell line U937 was plated onto chamber slides coated with recombinant human FN-1 with 5 µg/mL of the indicated antibodies and incubated for 1 hour at 37°C. Media was removed and cells were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with 1X Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) and then stained with 5 µg/mL of Ultra-LEAF™ purified anti-human CD157 (clone W21007F) (red), 5 µg/mL of Alexa Fluor® 647 anti-human CD29 (clone TS2/16) (blue) and Flash Phalloidin™ Green 488 (Cat. No. 424201) (green). Cells were washed 3 times with 1X Intracellular Staining Permeabilization Wash Buffer and then stained with Alexa Fluor® 555 anti-rat IgG (Cat. No. 405420) for 1 hour and imaged using a 40x objective.
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382105 100 µg 325 CHF
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Description

CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a GPI-anchored glycoprotein. It is an ectoenzyme that has both cyclic ADP-ribose hydrolase and ADP-ribosyl cyclase activities. It is prevalently expressed by cells of the myelomonocytic lineage, and modulates adhesion of neutrophils and monocytes to the extracellular matrix. CD157 is expressed in 97% of primary acute myeloid leukemia samples, and is used as a marker for diagnosis of Paroxysmal Nocturnal Hemoglobinuria, a disease caused by intrinsic deficiency of GPI anchors. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
hCD157 stable transfectant followed by recombinant protein (aa29-293)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, Activ, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For blocking of monocyte adhension, a concentration range of 1.25 – 5.0 µg/mL is suggested. For activation of signaling, a concentration range of 2.5 – 10.0 μg/mL is recommended. For immunocytochemistry, the suggested use of this reagent is 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This clone blocks adhesion of monocytes to fibronectin.

This clone activates calcium signaling in monocytes and induces cell polarization and actin polymerization. 

RRID
AB_2936535 (BioLegend Cat. No. 382105)

Antigen Details

Structure
GPI-anchored, ADP-ribosyl cyclase
Distribution

Cell Membrane

Function
ADP-ribosyl cyclase activity. Fibronectin, fibrinogen, laminin-1 and type 1 collagen binding. Hydrolase, Transferase.
Interaction
CD11b/CD18, ECM components
Ligand/Receptor
NAD, NADP
Cell Type
Antigen-presenting cells, Granulocytes, Monocytes
Biology Area
Cell Adhesion, Cell Proliferation and Viability, Innate Immunity
Molecular Family
CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References
  1. Ortolan E, et al. 2019. Immunology Letters. 205:59-64
  2. Ferrero E, et al. 2017. Sci Rep. 7: 15923.
  3. Kaisho T, et al. 1994. Proc Natl Acad Sci USA. 91:5325-9.
  4. Morone S, et al. 2014. J Biol Chem. 289:15588-601.
Gene ID
683 View all products for this Gene ID
UniProt
View information about CD157 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12.13.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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