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TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) Recombinant Antibody

Pricing & Availability
Clone
QA19A30 (See other available formats)
Regulatory Status
RUO
Other Names
CD16, CD32
Isotype
Human IgG4, κ
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Product Citations
publications
QA19A30_TruStain_CD16_CD32_Antibody_041422
C57BL/6 splenocytes were incubated with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) recombinant (clone QA19A30) (filled histogram) (0.25µg/106 cells, filled histogram) to block the Fc receptors, or were left untreated (black line histogram), then stained with anti-mouse CD90.2 (Thy1.2) FITC. Note the high background staining in the untreated cells.
  • QA19A30_TruStain_CD16_CD32_Antibody_041422
    C57BL/6 splenocytes were incubated with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) recombinant (clone QA19A30) (filled histogram) (0.25µg/106 cells, filled histogram) to block the Fc receptors, or were left untreated (black line histogram), then stained with anti-mouse CD90.2 (Thy1.2) FITC. Note the high background staining in the untreated cells.
Cat # Size Price Quantity Check Availability Save
163403 50 µg 65 CHF
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163404 500 µg 276 CHF
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Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Human
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) for 5 - 10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µL, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.

RRID
AB_2922498 (BioLegend Cat. No. 163403)
AB_2922498 (BioLegend Cat. No. 163404)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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