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True-Phos™ Perm Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Phospho-protein Fixation/Permeabilization Buffer Set
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Product Citations
publications
True_Phos_Perm_Buffer_050616
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, and intracellularly stained with anti-STAT6 Phospho (Tyr641) (clone A15137E) PE.
  • True_Phos_Perm_Buffer_050616
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, and intracellularly stained with anti-STAT6 Phospho (Tyr641) (clone A15137E) PE.
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425401 125 mL 159 CHF
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Description

True-Phos™ Perm Buffer has been developed to permeabilize fixed peripheral blood mononuclear cells (PBMCs), lysed whole blood, cell lines, or splenocytes. Cells can be stimulated cytokines (e.g. IL-4, IL-6, etc.), antibodies (e.g. anti-CD3, clone HIT3a), or phorbol-12-myristate 13-acetate and ionomycin (Cell Activation Cocktail; Cat. No. 423301), and fixed with Fixation Buffer (Cat. No. 420801), or for whole blood, RBC Lysis/Fixation Solution (Cat. No. 422401). This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.). Not all cell surface markers are compatible with BioLegend’s True-Phos™ Perm Buffer.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C, protected from prolonged exposure to light.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this product is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Use 1 mL of True-Phos™ Perm Buffer per 1 million cells, or 100 µL of whole blood.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to study phosphorylation kinetics in our Scientific Poster Library.

Product Citations
  1. Ryan C, et al. 2016. Blood. 128(25):2899-2908. PubMed

Antigen Details

Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins
Gene ID
NA

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Go To Top Version: 3    Revision Date: 05.20.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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