Treg-Protector™ (anti-ARTC2 Nanobody)

Pricing & Availability
Clone
S+16a (See other available formats)
Other Names
ART2.2, Art2b, Art, Rt6, Rt-6, Ly92b, Rt6-2
Isotype
Llama VH Ig domain
Ave. Rating
1 reviews
Product Citations
publications
S-16a_Treg-Protector_Block_1_020215
A C57BL/6 mouse was injected (i.v.) with 50 µg of Treg-Protector™ 15 minutes before harvesting the spleen to isolate lymphocytes (top). The same procedure was done with a control mouse that only received PBS (bottom). The cells obtained from each mouse were incubated for one hour at 37°C and then were stained with CD3 Brilliant Violet 605™, CD4 FITC, CD25 PE/Dazzle™ 594, and Annexin V Brilliant Violet 421™. Data shown was gated on the CD3+, and CD4+ population. Note the higher frequency of live Tregs (CD4+, CD25+, and Annexin V- T cells) in the mouse that was treated with Treg-Protector™ (top).
  • S-16a_Treg-Protector_Block_1_020215
    A C57BL/6 mouse was injected (i.v.) with 50 µg of Treg-Protector™ 15 minutes before harvesting the spleen to isolate lymphocytes (top). The same procedure was done with a control mouse that only received PBS (bottom). The cells obtained from each mouse were incubated for one hour at 37°C and then were stained with CD3 Brilliant Violet 605™, CD4 FITC, CD25 PE/Dazzle™ 594, and Annexin V Brilliant Violet 421™. Data shown was gated on the CD3+, and CD4+ population. Note the higher frequency of live Tregs (CD4+, CD25+, and Annexin V- T cells) in the mouse that was treated with Treg-Protector™ (top).
  • S-16a_Treg-Protector_Block_2_020215
See supplemental data
Cat # Size Price Quantity Avail. Save
149801 250 µg 295 CHF
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149802 1 mg 695 CHF
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Description

ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Recombinant
Immunogen
Expression vector encoding full length mouse ARTC2.
Formulation
1 mg/mL S+16a Nanobody in PBS. Purity ≥ 95%, as determined by Coomassie stained SDS-PAGE. Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.
Preparation
Treg-Protector ™ is sterilized by filtration through a 0.22 µm membrane and contains no preservatives.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C. Do not freeze. Keep sterile.
Application

Block - Quality tested

Recommended Usage

We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).

Application Notes

Treg-Protector™ (S+16a Nanobody) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a nanobody has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells8.

Application References

(PubMed link indicates BioLegend citation)
  1. Rissiek B, et al. 2014. J. Leuko. Biol. 95:543.
  2. Bannas P, et al. 2014. Contrast Media Mol. Imaging 9:135.
  3. Lischke T, et al. 2013. Eur. J. Immunol. 43:1828.
  4. Hubert S, et al. 2010. J. Exp. Med. 207:2561.
  5. Scheuplein F, et al. 2010. J. Autoimmun. 34:145.
  6. Scheuplein F, et al. 2009. J. Immunol. 182:2898.
  7. Koch-Nolte F, et al. 2007. FASEB J. 21:3490.
  8. Fernandez-Ruiz D, et al. 2016. Immunity. 45:889.
RRID
AB_2565485 (BioLegend Cat. No. 149801)
AB_2565494 (BioLegend Cat. No. 149802)

Antigen Details

Structure
GPI-anchored surface protein, 35 kD
Distribution

Subsets of mature T cells, regulatory T cells (Tregs), natural killer T cells (NKTs), and γδ T cells.

Function
ADP-ribosyltransferase.
Interaction
P2X7
Cell Type
T cells, Tregs, NKT cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References

1. Menzel S, et al. 2013. FEBS J. 280:3543.
2. Bannas P, et al. 2011. Mol. Immunol. 48:1762.
3. Chen YG, et al. 2011. J. Immunol. 186:4278.
4. Adriouch S, et al. 2007. J. Immunol. 179:186.
5. Bannas P, et al. 2005. Blood 105:3663.

Gene ID
11872 View all products for this Gene ID
UniProt
View information about ARTC2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.27.2016

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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