Loading...

Spark NIR™ 685 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
11-26c2a_SparkNIR685_IgD_Antibody_081220
C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse IgD (clone 11-26c.2a) Spark NIR™ 685 (left) or anti-mouse CD45R/B220 FITC only (right).
  • 11-26c2a_SparkNIR685_IgD_Antibody_081220
    C57BL/6 splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse IgD (clone 11-26c.2a) Spark NIR™ 685 (left) or anti-mouse CD45R/B220 FITC only (right).
Compare all formats
Cat # Size Price Quantity Check Availability Save
405749 25 µg 112 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
405750 100 µg 265 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2888693 (BioLegend Cat. No. 405749)
AB_2888693 (BioLegend Cat. No. 405750)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.12.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse IgD

  • PE anti-mouse IgD

  • Purified anti-mouse IgD

  • PerCP anti-mouse IgD

  • Biotin anti-mouse IgD

  • Brilliant Violet 711™ anti-mouse IgD

  • Alexa Fluor® 700 anti-mouse IgD

  • Alexa Fluor® 647 anti-mouse IgD

  • PerCP/Cyanine5.5 anti-mouse IgD

  • Pacific Blue™ anti-mouse IgD

  • APC anti-mouse IgD

  • APC/Cyanine7 anti-mouse IgD

  • Alexa Fluor® 488 anti-mouse IgD

  • PE/Cyanine7 anti-mouse IgD

  • Brilliant Violet 650™ anti-mouse IgD

  • Brilliant Violet 510™ anti-mouse IgD

  • Brilliant Violet 421™ anti-mouse IgD

  • Brilliant Violet 605™ anti-mouse IgD

  • Purified anti-mouse IgD (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse IgD

  • PE/Dazzle™ 594 anti-mouse IgD

  • APC/Fire™ 750 anti-mouse IgD

  • TotalSeq™-A0571 anti-mouse IgD

  • TotalSeq™-C0571 anti-mouse IgD

  • Spark NIR™ 685 anti-mouse IgD

  • TotalSeq™-B0571 anti-mouse IgD Antibody

  • Spark Violet™ 423 anti-mouse IgD

  • PE/Cyanine5 anti-mouse IgD

  • Brilliant Violet 785™ anti-mouse IgD
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account