Spark NIR™ 685 anti-human CD45RA Antibody, CD45RA, HI100

Pricing & Availability

Clone: HI100 (See other available formats)
Regulatory Status: RUO
Workshop: IV N906
Other Names: GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype: Mouse IgG2b, κ
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Product Citations: publications

HI100_SparkNIR685_CD45RA_Antibody_012420 — Human peripheral blood lymphocytes were stained with CD45RO PE and CD45RA (clone HI100) Spark NIR™ 685 (left) or CD45RO PE alone (right).

Compare all formats See Spark NIR™ 685 spectral data

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Size

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304167

25 tests

165 CHF

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304168

100 tests

399 CHF

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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions², immunohistochemical staining of frozen tissue sections³ and formalin-fixed paraffin-embedded tissue sections⁴, and immunocytochemistry^15,16.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
Palendira U, et al. 2008. Blood (FC) PubMed
Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
Canque B, et al. 2000. Blood 96:3748. (ICC)
Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2860793 (BioLegend Cat. No. 304167)
AB_2832590 (BioLegend Cat. No. 304168)

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution: B cells, naïve T cells, monocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RA on UniProt.org

Documentation

Reviews

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PE/Cyanine7 anti-human CD45RA	HI100	FC
PE/Dazzle™ 594 anti-human CD45RA	HI100	FC
APC/Fire™ 750 anti-human CD45RA	HI100	FC
Spark UV™ 387 anti-human CD45RA	HI100	FC
GMP APC anti-human CD45RA	HI100	FC
TotalSeq™-Bn0063 anti-human CD45RA	HI100	SB
Spark Blue™ 550 anti-human CD45RA	HI100	FC
GMP PE/Dazzle™ 594 anti-human CD45RA	HI100	FC
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Spark PLUS UV™ 395 anti-human CD45RA	HI100	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 01.23.2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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