Recombinant Mouse IL-22 (ELISA Std.)

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Other Names
Interleukin-22, IL-TIF
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Description

IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor).  IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29.  Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1).  Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Source
Mouse IL-22, amino acids Leu34-Val179 (Accessio # NM_016971) was expressed in E. coli.
Molecular Mass
The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kD. The DTT-reduced protein migrates at approximately 15 kD and the non-reduced protein migrates at approximately 13 kD by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant mouse IL-22 at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes; vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer, and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 3.9 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-22 protein is useful as a standard in a mouse IL-22 sandwich ELISA, using unlabeled poly5164 antibody (Cat. No. 516401) for capture and biotinylated poly5164 antibody (Cat. No. 516407) for detection.

Antigen Details

Biology Area
Stem Cells, Immunology, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dumoutier L, et al. 2000. J. Immun. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure.
4. Kotenko SV, et al. 2001. J. Immun. 166:7096.
5. Chang C, et al. 2006. Cell Research 16:902.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02.29.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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