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- Regulatory Status
- RUO
- Other Names
- Neurotrophic tyrosine kinase receptor type 2, TrkB, GP145-TrkB
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human NTRK2 binds to immobilized recombinant human BDNF in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 80 – 400 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 557502 | 10 µg | 112 CHF | ||||
| 557506 | 100 µg | 581 CHF | ||||
NTRK2 (Neurotrophic Tyrosine Kinase, Receptor, type 2), also known as TrkB (Tropomyosin related kinase B), is one of the members of the neurotrophin receptor family, which include TrkA and TrkC. All three Trk proteins exhibit a conserved subdomain that contains a signal peptide, two cysteine rich domains, a cluster of three leucine rich motifs, and two Ig-like domains in the extracellular portion. It has been shown that Trk receptor-mediated neurotrophic signaling regulates neuron physiology, cell proliferation and survival, axon and dendrite growth and patterning, and synaptic plasticity. The neurotrophins that activate NTRK2 are BDNF (Brain Derived Neurotrophic Factor), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4). BDNF is known to trigger NTRK2 dimerization and auto phosphorylation that leads to the activation of downstream signaling pathways. After the receptor-ligand binding, NTRK2-BDNF complex is internalized and transported from the nerve terminal to the cell body as signaling endosomes to mediate numerous signaling events. It has been demonstrated that activation of NTRK2 can lead to the down regulation of the main potassium-chloride cotransporter KCC2 in neurons, thereby increasing the intracellular Cl- concentration in lamina I neurons. Recently, it was shown that BDNF-NTRK2 signaling may play a role in the pathogenesis of post-traumatic stress disorders and mutations in the NTRK2 gene have been associated with obesity and mood disorders.
Product DetailsProduct Details
- Source
- Human NTRK2, amino acids (Cys32-His430) (Accession# NP_001007098), was expressed in 293E cells with a C-terminal FC and His tag.
- Molecular Mass
- The 639 amino acid recombinant protein has a predicted molecular mass of approximately 71 kD. The protein migrates approximately at 125 kD in DTT-reducing conditions and at approximately 250 kD in a non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Cys.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human NTRK2 binds to immobilized recombinant human BDNF in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 80 – 400 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer.
- Distribution
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NTRK2 is found primarily in the nervous system. It also can be found in the pancreas, skeletal muscles, and the kidney. A truncated form of TrkB (missing the tyrosine kinase domain) is found in heart, ovary, and the spleen.
- Function
- NTRK2, when binded to BDNF, NT-3, and NT-4, is able to exert neuron cells' biological functions such as proliferation, survival, axon, and dendrite growth.
- Interaction
- Neuron cells
- Ligand/Receptor
- BDNF, NT-3, and NT-4.
- Bioactivity
- NTRK2 is able to bind BDNF in a dose-dependent manner.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Protein Kinases/Phosphatase, Soluble Receptors
- Antigen References
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1. Snider WD. 1994. Cell 77:627.
2. Grace PM, et al. 2014. Nat. Rev. Immunol. 14:217.
3. Neveu I and Arenas E. 1996. J. Cell Biol. 133:631.
4. Green CR, et al. 2013. J. Depress Anxiety S4:006.
5. Gupta VK, et al. 2013. Int. J. Mol. Sci. 14:10122.
6. Pla P, et al. 2014. Front Behav. Neurosci. 8:135.
7. Liu X, et al. 2014. J. Biol. Chem. 289:27571. - Gene ID
- 4915 View all products for this Gene ID
- UniProt
- View information about NTRK2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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