Recombinant Human IL-6 (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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570809 4 pack 80,00 CHF
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. Recombinant human IL-6 is a 26 kD protein containing 184 amino acids.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
The mature form of human IL-6, amino acids Pro 29-Met 212 (Accession # NM_000600) was expressed in E. coli.
Molecular Mass
The 185 amino acid N-terminal methionylated recombinant protein has a predicted molcular mass of 21,041 Da. The DTT-reduced and the non-reduced protein migrates at approximately 23kDa by SDS-PAGE.
Purity
Purity is >98% as determined by SDS PAGE , Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 8 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-6 protein is useful as a standard for a human IL-6 sandwich ELISA, using unlabeled MQ2-13A5 antibody (catalog #501102) for capture and biotinylated MQ2-39C3 antibody (catalog #501202) for detection.

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Antigen Details

Structure
Neuropoietic cytokine family
Distribution

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Function
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction
T cells, B cells, hepatocytes, cholinergic neurons
Ligand/Receptor
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 11.03.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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