Purified Armenian Hamster IgG Isotype Ctrl Antibody

Pricing & Availability
Clone
HTK888 (See other available formats)
Regulatory Status
RUO
Isotype
Armenian Hamster IgG
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Product Citations
publications
1_HTK888_Purified_IgG_Antibody_021920
Whole cell extracts (190 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of purified Armenian Hamster IgG isotype ctrl antibody (clone HTK888) (Cat. No. 400902) or purified anti-mouse/human Helios antibody (clone 22F6) (Cat. No. 137202). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit antibody against human Helios overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (minimal x-reactivity) antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular weight marker.
  • 1_HTK888_Purified_IgG_Antibody_021920
    Whole cell extracts (190 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of purified Armenian Hamster IgG isotype ctrl antibody (clone HTK888) (Cat. No. 400902) or purified anti-mouse/human Helios antibody (clone 22F6) (Cat. No. 137202). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit antibody against human Helios overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (minimal x-reactivity) antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular weight marker.
  • 2_HTK888_GoChIP_IgG_Antibody_1_082619.png
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 3 µg of chromatin and either 1.2 µg of Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Control Antibody (clone HTK888, in black) or no antibody (beads control, in gray). Protein G beads were used to isolate immune complexes. The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for active (human GAPDH promoter and human RPL30 exon 3) and inactive (human α-Satellite repeats) loci. The amount of immunoprecipitated DNA in each sample is represented as percentage to the total amount of input chromatin, which is equivalent to 100%.
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400901 50 µg 40,00 CHF
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400902 500 µg 110,00 CHF
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Description

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC, ICFC - Quality tested
IP, ChIP - Verified
ICC, IHC, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717.
  2. Yu R, et al. 2006. Obesity 14:1353.
  3. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed
  4. Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed
  5. Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations
  1. Olcina MM et al. 2018. Cell reports. 25(13):3721-3732 . PubMed
  2. Bechelli J, et al. 2019. Infect Immun. 87:. PubMed
  3. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  4. Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
  5. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
  6. Nie S, et al. 2019. Cancer Manag Res. 11:10257. PubMed
  7. Lagarrigue F, et al. 2018. Blood Adv. 1.720833333. PubMed
  8. Yang J, et al. 2005. Rheumatology. 44:1245. PubMed
  9. Goldstein H 2009. J Am Soc Nephrol. 1.55. PubMed
  10. Sainson RCA, et al. 2020. Cancer Immunol Res. 1.422222222. PubMed
  11. Martinez LE, et al. 2020. J Virol. 94:00:00. PubMed

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Biotin Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
FITC Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
PE Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC, IP, ICC, IHC, WB, FA, ChIP
PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Pacific Blue™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
PerCP Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC, ICC, IHC, IP, WB, FA
Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl HTK888 ICC
APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC
GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC, ICFC, ICC, IHC, IP, WB, FA
Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Ctrl HTK888 ChIP, FC, ICFC
TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl HTK888 FC
TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
Go To Top Version: 4    Revision Date: 08.24.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Armenian Hamster IgG Isotype Ctrl

  • Biotin Armenian Hamster IgG Isotype Ctrl

  • FITC Armenian Hamster IgG Isotype Ctrl

  • PE Armenian Hamster IgG Isotype Ctrl

  • PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl

  • Purified Armenian Hamster IgG Isotype Ctrl

  • PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl

  • Pacific Blue™ Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl

  • APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl

  • PerCP Armenian Hamster IgG Isotype Ctrl

  • PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl

  • Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl

  • PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl

  • APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl

  • GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl

  • Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl

  • KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl

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