Purified anti-TCP-1β Antibody

Pricing & Availability
Clone
F39P7F11 (See other available formats)
Regulatory Status
RUO
Other Names
CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_F39P7F11_Pure_TCP-1B_Antibody_113018
Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_F39P7F11_Pure_TCP-1B_Antibody_113018
    Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_TCP-1beta_F39P7F11_111010
    HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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635502 100 µg 195,00 CHF
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Description

TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kD, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Frog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide RKRVPDHHPC
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
Product Citations
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
RRID
AB_2275598 (BioLegend Cat. No. 635502)

Antigen Details

Structure
Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution

Cytoplasm

Function
In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
Interaction
β-actin, 14-3-3γ, SUMO4, TCP-1α, β-Tubulin, α-tubulin, CCT8
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References

1. Yaffe MB, et al. 1992. Nature 358:245

Gene ID
21454 View all products for this Gene ID
UniProt
View information about TCP-1 beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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