- SP10 (See other available formats)
- Regulatory Status
- Other Names
- Vesicle-associated membrane protein 1, synaptobrevin-1
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|837003||25 µg||90,00 CHF|
|837004||100 µg||235,00 CHF|
Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- This antibody was raised against a crude synaptic preparation of post mortem human brain.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Verified
ELISA, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.
(PubMed link indicates BioLegend citation)
- Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
- Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
- Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
- Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed
- Product Citations
AB_2810717 (BioLegend Cat. No. 837003)
AB_2810718 (BioLegend Cat. No. 837004)
- Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Tissue distribution: Nervous system, skeletal muscle and adipose tissue.
Cellular distribution: Synaptic vesicle membrane, cytoplasmic vesicle membrane, and mitochondrion
- Synaptobrevin is involved in the targeting and/or fusion of transport vesicles to their target membrane.
- VAPA, VAPB, Syntaxin, SNAP25
- Cell Type
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
- Gene ID
- 6843 View all products for this Gene ID
- View information about Synaptobrevin on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.