Purified anti-SIRT2 Antibody

Pricing & Availability
Clone
W16205A (See other available formats)
Other Names
Sirtuin 2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2 (SIR2L2), SIR2L
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16205A_PURE_SIRT2_Antibody_120417
Total protein from human brain (5 µg, lane 1), mouse brain (10 µg, lane 2), rat brain (10 µg, lane 3) and 15µg of total protein from NTERA2 (lane 4), HeLa (lane 5), EL-4 (lane 6) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:1000 diluted (0.5 µg/mL) and 1:2000 diluted (0.25 µg/mL) purified anti-SIRT2 antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (Cat. No. 405405) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16205A_PURE_SIRT2_Antibody_120417
    Total protein from human brain (5 µg, lane 1), mouse brain (10 µg, lane 2), rat brain (10 µg, lane 3) and 15µg of total protein from NTERA2 (lane 4), HeLa (lane 5), EL-4 (lane 6) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL), 1:1000 diluted (0.5 µg/mL) and 1:2000 diluted (0.25 µg/mL) purified anti-SIRT2 antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (Cat. No. 405405) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
650201 25 µg 85 CHF
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650202 100 µg 215 CHF
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Description

Mammalian sirtuin family proteins are homologs of the Saccharomyces cerevisiae silencing protein Sir2. Sirtuins are NAD-dependent deacylases and ADP-ribosyl transferases involved in the regulation of various cellular processes, such as metabolism, autophagy, apoptosis, gene silencing, and lifespan. Unlike the other sirtuin members, Sirtuin 2 (SIRT2) is predominantly distributed in the cytoplasm and functions as a microtubule deacetylase. SIRT2 also deacetylates lysines on histone and several key transcriptional factors, including P53 and NF-κB. SIRT2 shuttles continuously between cytoplasmic and nuclear during interphase, associates with chromatin in early prophase, and localizes to centrosomes and the mitotic spindle during metaphase. SIRT2 has been implicated in the pathology of cancer and neurodegenerative diseases. SIRT2 deacetylates histone proteins and prevents chromosomal instability during mitosis. Inhibition of SIRT2 has been shown to negatively regulate polyglutamine inclusion and rescue neuronal cells from α-synuclein-induced toxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human SIRT2 recombinant protein (140-389 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

As per the Human Protein Atlas RNA level dataset, NTERA2 cell is expected to have a low level of SIRT2 expression.

Human SIRT2 has 5 isoforms with Mw ranging from 30kDa to 43kDa.

This clone cannot be used for IF application (in house tested).

RRID
AB_2721752 (BioLegend Cat. No. 650201)
AB_2721753 (BioLegend Cat. No. 650202)

Antigen Details

Structure
SIRT2 have five reported isoforms, with the predicted molecular weight of 43.2, 41.3, 39.5, 35.7, and 30.4 kD. Contains a sirtuin-type deacetylase domain.
Distribution

Cytoplasm, Nucleus. 

Function
Sirtuin 2 (SIRT2) is an NAD-dependent deacetylase known to participates in the modulation of multiple biological processes, including cell cycle progression, genomic stability, microtubule dynamics, NAD metabolism, and autophagy.
Interaction
Interacts with CDC20, FOXO3, FZR1, HDAC6, FOXO1, RELA, HOXA10, YWHAB, YWHAG, ERK1/2, KMT5A, G6PD, AURKA, CDK5R1, CDK5, SARS, and HIF1A.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Jęśko H, et al. 2017. Neurochem Res. 42:876.
2. Cosentino C, et al. 2014. EMBO J. 33:1417.
3. Nagai T, et al. 2013. J Cell Sci. 126:4369.
4. Donmez G, et al. 2013. EMBO Mol Med. 5:344.
5. Cha YI, et al. 2013. BMB Rep. 46:429.
6. Inoue T, et al. 2007. Cell Cycle. 6:1011.

Gene ID
22933 View all products for this Gene ID
UniProt
View information about SIRT2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12.05.2017

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