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Purified anti-Pax-6 Antibody (Previously Covance catalog# PRB-278P)

Pricing & Availability
Clone
Poly19013 (See other available formats)
Regulatory Status
RUO
Other Names
Paired box protein Pax-6, oculorhombin, aniridia type II protein, Sey, Protein eyeless, PRB-278P-100
Previously
Covance Catalog# PRB-278P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1-Poly19013_PURE_Pax-6_Antibody_1_091420
Whole cell extracts (15µg total protein) from 293T (human), (left panel) or E17 Ms. brain (mouse brain at day 17 of development), (right panel) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF membrane and probed with 1.0 µg/mL (1:2000 dilution) of purified Pax-6 rabbit antibody (clone Poly19013) for 2 hr at RT. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at 1:25000 dilution (lower). Lane M:Molecular weight marker.
  • 1-Poly19013_PURE_Pax-6_Antibody_1_091420
    Whole cell extracts (15µg total protein) from 293T (human), (left panel) or E17 Ms. brain (mouse brain at day 17 of development), (right panel) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF membrane and probed with 1.0 µg/mL (1:2000 dilution) of purified Pax-6 rabbit antibody (clone Poly19013) for 2 hr at RT. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at 1:25000 dilution (lower). Lane M:Molecular weight marker.
  • 2-Poly19013_PURE_Pax-6_Antibody_2_091420
    Whole cell extracts (15 µg total protein) from either untransfected 293E cells or transfected with GFP were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF membrane and probed with 1.0 µg/mL (1:2000 dilution) of purified Pax-6 rabbit antibody (clone Poly19013) (panel A) or purified anti-GFP antibody (clone 1GFP63, Cat. No. 668206) for 2 hr at RT. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401, 1:3000 dilution) or HRP Goat anti-mouse IgG Antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at 1:25000 dilution (lower).Lane M:Molecular weight marker.
  • 3_Purified_Pax-6_Antibody_IHC_2_021315
    IHC staining of purified anti-Pax-6 antibody (clone Poly19013) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 20 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Check Availability Save
901302 25 µL 136 CHF
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901301 100 µL 413 CHF
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Description

Pax6 is a transcription factor present during embryonic development. The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. It is a key regulatory gene of eye and brain development. Within the brain, the protein is involved in development of specialized cells that process smell. Pax-6 acts as a critical gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. As a transcription factor, Pax6 acts at the molecular level in the signaling and formation of the central nervous system. The characteristic paired DNA binding domain of Pax6 utilizes two DNA-binding domains, the paired domain (PD), and the paired-type homeodomain (HD). These domains function separately. An example of this lies in HD’s regulatory involvement in the formation of the lens and retina throughout oculogenesis contrasted by the molecular mechanisms of control exhibited on the patterns of neurogenesis in brain development by PD. The HD and PD domains act in close coordination, giving Pax6 its multifunctional nature in directing molecular signaling in formation of the CNS.

The vertebrate PAX6 locus encodes at least three different protein isoforms, these being the canonical PAX6, PAX6(5a), and PAX6(ΔPD). The canonical PAX6 protein contains an N-terminal paired domain, connected by a linker region to a paired-type homeodomain, and a proline/serine/threonine (P/S/T)-rich C-terminal domain. The paired domain and paired-type homeodomain each have DNA binding activities, while the P/S/T-rich domain possesses a transactivation function. PAX6(5a) is a product of the alternatively spliced exon 5a resulting in a 14 residue insertion in the paired domain which alters the specificity of this DNA binding activity. The nucleotide sequence corresponding to the linker region encodes a set of three alternative translation start codons from which the third PAX6 isoform originates. Collectively known as the PAX6(ΔPD) or pairedless isoforms, these three gene products all lack a paired domain. The pairedless proteins possess molecular weights of 43, 33, or 32kDa, depending on the particular start codon used. PAX6 transactivation function is attributed to the variable length C-terminal P/S/T-rich domain which stretches to 153 residues in human and mouse proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was generated against the peptide (QVPGSEPDMSQYWPRLQ) derived from the C-terminus of the mouse Pax-6 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC-P - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and formalin-fixed paraffin-embedded immunohistochemical staining of brain tissue. For Western blotting, the suggested use of this reagent is 1.0 µg/mL (1:2000). For immunohistochemistry, a dilution of 1:50 - 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody weakly reacts with rat. It has also shown reactivity with GFP and is therefore not recommended for use in GFP-expressing systems (as determined by in-house testing).

Pax-6 has two isoforms in human and mice at 46.6 and 48.2 Kd. This antibody recognizes both. The sequence is highly conserved among Pax-6 of various species. The antibody was subsequently purified on a Protein A column and is useful in studying brain, neuronal and olfactory development in higher eukaryotes.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Tang K, et al. 2012. Development. 139:1630. (IF) PubMed
  2. Bandah D, et al. 2007. Invest. Ophthalmol. Vis. Sci. 48:2503. (WB) PubMed
  3. Marquardt T, et al. 2001. Cell. 105:43.
  4. Yamamoto Y, Jeffery WR. 2000. Science. 289:631
  5. Ashery-Padan R, et al. 2000. Genes Dev. 14:2701.
  6. Osumi N, et al. 1997. Development. 124:2961.
  7. Koroma BM, et al. 1997. Invest. Ophthalmol Vis Sci. 38:108.
  8. Davis JA, Reed RR. 1996. J Neurosci. 16:5082. (IHC)
  9. Quadrato G, et al. Nature. 545:48. (IF) PubMed
Product Citations
  1. Scior A, et al. 2018. EMBO J. 37:282. PubMed
  2. Wilson CS, et al. 2019. JCI Insight. 5. PubMed
  3. Nazir FH, et al. 2018. Neurochem Int. 121:38. PubMed
  4. Bashford AL, et al. 2019. J Pathol. 248:396. PubMed
  5. Del Toro D et al. 2017. Cell. 169(4):621-635 . PubMed
  6. Ramzy A, et al. 2018. Endocrinology. 159:83. PubMed
  7. Takahashi K, et al. 2021. PLoS Genet. 17:e1009587. PubMed
  8. Ziffra RS, et al. 2021. Nature. 598:205. PubMed
  9. Schroeter MS, et al. 2022. MRS Bull. 47:530. PubMed
  10. Ahmad Z, et al. 2015. PLoS One. 10: 0144597. PubMed
  11. Subramanian L, et al. 2017. Nat Commun. 8:14167. PubMed
  12. Schmid B, et al. 2021. Stem Cell Research. 52:102180. PubMed
  13. Ma L, et al. 2021. Stem Cell Research. 53:102305. PubMed
  14. Li J, et al. 2021. Stem Cell Research. 53:102376. PubMed
  15. Martens YA, et al. 2021. STAR Protocols. 2(2):100571. PubMed
  16. Hwang SH, et al. 2021. eLife. 0.416666666666667. PubMed
  17. Tristan CA, et al. 2020. bioRxiv. . PubMed
  18. Bershteyn M, et al. 2017. Cell Stem Cell. 20:435. PubMed
  19. Panagiotakos G, et al. 2019. Elife. 8:e51037. PubMed
  20. Peter CJ, et al. 2019. Nat Commun. 10:4112. PubMed
  21. Bovio PP, et al. 2019. Mol Neurobiol. 56:4273. PubMed
  22. Ing-Esteves S, et al. 2018. J Neurosci. 38:2713. PubMed
  23. Viais R, et al. 2021. Elife. 10:. PubMed
  24. Avilés EC, et al. 2022. Cell Rep. 38:110307. PubMed
  25. Notaras M, et al. 2021. Mol Psychiatry. Online ahead of print. PubMed
  26. Taniguchi-Ikeda M, et al. 2021. iScience. 24:103140. PubMed
  27. Merkert S, et al. 2021. Stem Cell Research. 52:102208. PubMed
  28. Gao X, et al. 2020. Stem Cell Research. 48:101986. PubMed
  29. Patmore DM, et al. 2020. Developmental Cell. 54(4):455-470.e5. PubMed
  30. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  31. Small K, et al. 2015. Ophthalmology. 13: 681-685. PubMed
  32. Hickmott JW, et al. 2018. Gene Ther. 25:524. PubMed
  33. Liu J et al. 2017. Cell stem cell. 21(5):635-649 . PubMed
  34. Bonnefont J, et al. 2019. Neuron. 103:1096. PubMed
  35. Junqueira Alves C, et al. 2021. Nat Commun. 12:6019. PubMed
  36. Moura D, et al. 2016. Sci Rep. 6:28532. PubMed
  37. Sun X, et al. 2021. Stem Cell Research. 52:102230. PubMed
  38. Kafarnik C, et al. 2020. Stem Cells Dev. 425:29. PubMed
  39. Sood S, et al. 2020. Proc Natl Acad Sci U S A. 22331:117. PubMed
  40. Singer RA, et al. 2020. Cell Metabolism. 30(6):1091-1106. PubMed
  41. Saito K, et al. 2019. Cell Rep. 29:1555. PubMed
  42. Girskis KM, et al. 2021. Neuron. 109:3239. PubMed
  43. Liu X, et al. 2021. Cell Prolif. 54:e13124. PubMed
  44. Kalusa M, et al. 2021. Front Neuroanat. 15:678385. PubMed
  45. Gregory JA, et al. 2020. Cells. 9:00. PubMed
  46. de Leeuw VC, et al. 2020. Cell Reprogram. 1.125. PubMed
  47. Ucuncu E, et al. 2020. Nat Commun. 4.685416667. PubMed
  48. Janssens S, et al. 2018. mSystems. 3. PubMed
  49. Tripathy R, et al. 2018. Neuron. 100:1354. PubMed
  50. Wang H, et al. 2019. Cell Rep. 27:2335. PubMed
  51. Chen Y, et al. 2019. Methods Mol Biol. 1919:59:00. PubMed
  52. Moore D, et al. 2019. Stem Cells Int. 2019:8710180. PubMed
  53. Komabayashi-Suzuki M, et al. 2019. Cell Rep. 29:1113. PubMed
  54. Selvadurai HJ, et al. 2020. Cell Reports. 31(2):107511.. PubMed
  55. Shao W, et al. 2020. Nature. 580:106. PubMed
  56. LaForce GR, et al. 2022. Neuron. 110:1340. PubMed
  57. Tristan CA, et al. 2022. Nat Protoc. Online ahead of print. PubMed
  58. Bandah D, et al. 2007. Invest Ophthalmol Vis Sci. 48:2503-2509. PubMed
  59. Pataskar A, et al. 2016. EMBO J. 35: 24 - 45. PubMed
  60. Broix L, et al. 2016. Nat Genet. 48:1349-1358. PubMed
  61. Slot E, et al. 2020. Stem Cell Res. 101745:44. PubMed
  62. Sun WR, et al. 2020. Hum Mol Genet. . PubMed
  63. Freude KK, et al. 2020. World J Stem Cells. 1171:12. PubMed
  64. Ezan J, et al. 2021. Sci Rep. 9106:11. PubMed
  65. Nakashima H, et al. 2021. Cell Reports. 35(7):109124. PubMed
  66. Lardelli R, et al. 2017. Nat Genet. 10.1038/ng.3762. PubMed
  67. Taverna E, et al. 2016. Sci Rep. 6:21206. PubMed
  68. Shafa M, et al. 2018. Front Med (Lausanne). 9:1816. PubMed
  69. Zhang X, et al. 2019. Protein Cell. 10:649. PubMed
  70. Postel M, et al. 2019. BMC Bioinformatics. 20:470. PubMed
  71. Matsumura K, et al. 2020. Nat Commun. 11:859. PubMed
  72. Lee EJ, et al. 2020. Cell Reports. 28(3):712-722.e3.. PubMed
  73. Roy A, et al. 2020. Acta Neuropathologica Communications. 8(1):54.. PubMed
  74. Simsa R, et al. 2021. PLoS One. 16:e0245685. PubMed
  75. Li M, et al. 2021. Nat Commun. 12:420. PubMed
  76. Craenen K, et al. 2020. Reprod Toxicol. 91:59. PubMed
  77. Walker H, et al. 2021. Int J Mol Sci. 22:. PubMed
  78. Hou S, et al. 2021. Cereb Cortex. 31:4730. PubMed
  79. Ma L, et al. 2022. iScience. 25:105172. PubMed
  80. Delépine C, et al. 2016. Hum Mol Genet. 25: 146 - 157. PubMed
  81. Xu J, et al. 2016. Sci Transl Med. 8: 333ra48. PubMed
  82. Bonney S, et al. 2016. J Neurosci. 36: 7786 - 7801. PubMed
  83. Pei S, et al. 2016. Sci Rep. 6:33822. PubMed
  84. M Rodriguez, S Noctor, E Muñoz 2016. PLoS One. 11:e0167063. PubMed
  85. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  86. Ahn LY, et al. 2021. J Neurosci Res. 99:110. PubMed
  87. Birkhoff JC, et al. 2020. Hum Mol Genet. 29:2535. PubMed
  88. Pereiro X, et al. 2020. Cells. 9:00. PubMed
  89. Kim TW, et al. 2021. Cell Stem Cell. 28(2):343-355.e5. PubMed
  90. Tomita H, et al. 2020. Cell Reports. 30(1):215-228.e5.. PubMed
  91. Plaza Reyes A, et al. 2020. Nat Commun. 11:1609. PubMed
  92. Wang Q, et al. 2017. Curr Biol. 27:1791. PubMed
  93. Todd L, et al. 2017. Glia. 65:1640. PubMed
  94. Lu HC, et al. 2017. Nat Genet. 49:527. PubMed
  95. Dhumale P, et al. 2018. PLoS One. 9:1544. PubMed
  96. Chen X, et al. 2022. PLoS Biol. 20:e3001868. PubMed
  97. Li N, et al. 2021. Stem Cell Research. 53:102322. PubMed
  98. Gao X, et al. 2021. Stem Cell Research. 53:102271. PubMed
  99. Schinzel F, et al. 2021. Journal of Comparative Neurology. 529(11):3032-3045. PubMed
  100. Xiao D, et al. 2020. Sci Adv. 6:eaaz5858. PubMed
  101. Retallack H, et al. 2016. Proc Natl Acad Sci U S A. 113(50):14408-14413. PubMed
  102. Simonin Y, et al. 2016. EBioMedicine. 12:161-169. PubMed
  103. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  104. Sun D, et al. 2017. PLoS One. 12(6):e0179047. PubMed
  105. Chau KF, et al. 2018. Elife. 19:E663. PubMed
  106. Andres LM, et al. 2017. ACS Chem Biol. 12:2030. PubMed
  107. Ibañez Rodriguez MP et al. 2018. The Journal of comparative neurology. 526(15):2462-2481 . PubMed
  108. Pollen AA, et al. 2019. Cell. 176:743. PubMed
  109. Cliff TS et al. 2017. Cell stem cell. 21(4):502-516 . PubMed
  110. Fregeac J, et al. 2020. Mol Autism. 11:22. PubMed
  111. Li L, et al. 2019. Cell Rep. 28:698. PubMed
  112. Omer Javed A, et al. 2018. Cell Rep. 25:368. PubMed
  113. Marczenke M, et al. 2021. Development. 148:. PubMed
  114. Zhu X, et al. 2022. Sci Adv. 8:eabl7263. PubMed
  115. Harkin L, et al. 2016. Cereb Cortex. 10.1093/cercor/bhw394. PubMed
  116. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  117. Eze UC, et al. 2021. Nature Neuroscience. 24(4):584-594. PubMed
  118. Chen M, et al. 2021. Stem Cell Research. 51:102166. PubMed
  119. Ramme AP, et al. 2020. Stem Cell Research. 41:101615.. PubMed
  120. Popovitchenko T, et al. 2020. Nat Commun. 11:1674. PubMed
  121. Velasco S, et al. 2019. Nature. 570:523. PubMed
  122. Forrest MP, et al. 2017. Cell Stem Cell. 1.086805556. PubMed
  123. Lavado A et al. 2018. Dev Cell. 47(5):576-591 . PubMed
  124. Docampo–Seara A, et al. 2018. Brain Struct Funct. 223:3593. PubMed
  125. Langer LF, et al. 2019. Elife. 8. PubMed
  126. Hoshino A, et al. 2017. Dev Cell. 43:763. PubMed
  127. Luisier R, et al. 2018. Nat Commun. 8:6458. PubMed
  128. Haldipur P, et al. 2021. Acta Neuropathol. 142:761. PubMed
  129. Li J, et al. 2021. Neural Plast. 2021:4894881. PubMed
  130. Schembs L, et al. 2022. Cell Rep. 39:110811. PubMed
  131. Soubannier V, et al. 2022. Cells. 11:. PubMed
  132. Manos JD, et al. 2022. iScience. 25:103658. PubMed
  133. Han X, et al. 2021. J Neurosci. Online ahead of print. PubMed
  134. Urciuolo A, et al. 2020. Nat Biomed Eng. 4:901. PubMed
  135. Ramme AP, et al. 2021. Stem Cell Research. 53:102327. PubMed
  136. Zhang L, et al. 2020. Cell Stem Cell. 27(5):784-797.e11. PubMed
  137. Jeong D, et al. 2020. Cell Rep. 33:108257. PubMed
  138. Breuss M, et al. 2017. Hum Mol Genet. 10.1093/hmg/ddw383. PubMed
  139. Paquet D, et al. 2016. Nature. 533: 125-129. PubMed
  140. Goodson NB, et al. 2018. Dev Biol. 434:149. PubMed
  141. Ren X, et al. 2019. Cell Rep. 26:3643. PubMed
  142. Beattie R et al. 2017. Neuron. 94(3):517-533 . PubMed
  143. Parween S, et al. 2018. Front Cell Neurosci. 0.746527778. PubMed
  144. Wörsdörfer P, et al. 2019. Sci Rep. 9:15663. PubMed
  145. Pandey PR, et al. 2019. Int J Mol Sci. 0.936805556. PubMed
  146. Mirjalili Mohanna SZ, et al. 2020. Mol Ther Methods Clin Dev. 17:478. PubMed
  147. Yin WC, et al. 2019. Dev Cell. 48:167. PubMed
  148. Li YP, et al. 2021. STAR Protoc. 2:100438. PubMed
  149. Clark AE, et al. 2020. J Virol. 95:e02024. PubMed
  150. Chui A, et al. 2020. Development. . PubMed
  151. Chao J, et al. 2022. iScience. 25:104391. PubMed
  152. Okamoto M, et al. 2016. Nat Commun. 7: 11349. PubMed
  153. Hickmott J, et al. 2016. Mol Ther Methods Clin Dev. 3:16051. PubMed
  154. Zhu L, et al. 2016. J Cell Biol. 215: 187 - 202. PubMed
  155. Ren R, et al. 2017. Cell Res. 10.1038/cr.2017.18. PubMed
  156. Ao Z, et al. 2020. Anal Chem. 4630:92. PubMed
  157. Shu P, et al. 2019. J Biol Chem. 294:3444. PubMed
  158. Mao X, et al. 2019. Stem Cell Reports. 13:747. PubMed
  159. Lindsay CD, et al. 2019. Acta Biomater. 95:225. PubMed
  160. Shafa M, et al. 2019. Int J Mol Sci. 0.95. PubMed
  161. Gabriel E et al. 2017. Cell stem cell. 20(3):397-406 . PubMed
  162. Homman‐Ludiye J et al. 2018. The Journal of comparative neurology. 526(17):2870-2883 . PubMed
  163. Xu D, et al. 2018. PLoS Biol. 16:e2006613. PubMed
  164. Zahr SK et al. 2018. Neuron. 97(3):520-537 . PubMed
  165. Kim YJ, et al. 2018. Neuron. 100:1180. PubMed
  166. Tu J et al. 2018. Stem cell research. 28:29-32 . PubMed
  167. Mills T, et al. 2017. PLoS One.. 10.1371/journal.pone.0176905. PubMed
  168. Padmanabhan Nair V, et al. 2021. Cell Stem Cell. . PubMed
  169. Chui A, et al. 2020. Development. 147:00:00. PubMed
  170. Quintana-Urzainqui I, et al. 2020. Development. 147:00:00. PubMed
  171. Gey M, et al. 2016. J Neurosci. 36: 9057 - 9069. PubMed
  172. Sancho-Martinez I, et al. 2016. Nat Commun. 7:10743. PubMed
  173. Robson JP, et al. 2019. PLoS One. 14:e0210665. PubMed
  174. Remez LA, et al. 2017. Dev Biol. 432:140. PubMed
  175. Klein T et al. 2019. Stem cell research. 1724:56:00 . PubMed
  176. Sano N et al. 2017. Frontiers in cellular neuroscience. 0.54375 . PubMed
  177. Kaufman ML, et al. 2021. Development. . PubMed
  178. Romero DM, et al. 2022. Nat Commun. 13:2746. PubMed
  179. Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed
  180. Prince LM, et al. 2021. Food Chem Toxicol. 152:112178. PubMed
  181. Behesti H, et al. 2021. Elife. 10:. PubMed
  182. Garreta E, et al. 2016. Biomaterials. 98:64-78. PubMed
  183. Kim HM, et al. 2020. Cell Prolif. :e12965. PubMed
  184. Usman A, et al. 2021. Stem Cell Research. 55:102488. PubMed
  185. Cheng S, et al. 2019. J Neurosci. 39:2195. PubMed
  186. Uzquiano A, et al. 2019. Cell Rep. 28:1596. PubMed
  187. Docampo–Seara A, et al. 2019. Brain Struct Funct. 224:2325. PubMed
  188. Lim Y, et al. 2019. Sci Rep. 9:226. PubMed
  189. Nakano Y et al. 2018. Cell. 174(3):536-548 . PubMed
  190. Zhu Y, et al. 2017. Lab Chip. 10.1039/c7lc00682a. PubMed
  191. Metzger JJ, et al. 2022. Cell Rep Methods. 2:100297. PubMed
  192. Marinopoulou E, et al. 2021. iScience. 24:103198. PubMed
  193. Chai G, et al. 2020. Neuron. 109(2):241-256.e9. PubMed
  194. Yu Q, et al. 2021. eLife. 10:00. PubMed
  195. Miyashita H, et al. 2017. Sci Rep. 7:43557. PubMed
  196. Tachibana N, et al. 2016. J Neurosci. 36: 9454 - 9471. PubMed
  197. Lin H, et al. 2016. Nature. 531:323-328. PubMed
  198. Wang W, et al. 2016. Nat Commun. 7:10936. PubMed
  199. Bando Y, et al. 2016. Cereb Cortex. 26: 106 - 117. PubMed
  200. Wu X, et al. 2018. Cell. 172:423. PubMed
  201. Shibata S et al. 2018. Cell reports. 25(6):1668-1679 . PubMed
  202. Thomas Klein et al. 2018. Stem cell research. 33:171-174 . PubMed
  203. Ward JM, et al. 2019. Cell Rep. 26:1189. PubMed
  204. Wang S, et al. 2019. Protein Cell. 10:1. PubMed
  205. Watanabe Y, et al. 2018. Development. 145. PubMed
  206. Wu Z, et al. 2019. Neuron. 101:635. PubMed
  207. Miesfeld JB, et al. 2018. Sci Rep. 8:10195. PubMed
  208. Hasenpusch–Theil K, et al. 2020. eLife. 9:e58162. PubMed
  209. Bendriem RM, et al. 2020. eLife. 8:e49376.. PubMed
  210. Inak G, et al. 2021. Nat Commun. 12:1929. PubMed
  211. Liang D, et al. 2021. Nat Neurosci. 24:941. PubMed
  212. Vajhøj C, et al. 2021. PLoS One. 16:e0261536. PubMed
  213. Nieto-Estévez V, et al. 2022. Front Mol Neurosci. 14:815808. PubMed
  214. Nieto-Estévez V, et al. 2022. Front Mol Neurosci. 15:889922. PubMed
  215. Tang K, et al. 2012. Development. 139:1630-1639. PubMed
  216. He S, et al. 2016. Development. 143: 1937 - 1947. PubMed
  217. Lajko M, et al. 2016. PLoS One. 11:e0166886. PubMed
  218. Li M, et al. 2021. Signal Transduct Target Ther. 6:05. PubMed
  219. Kitajima R, et al. 2020. Stem Cell Res. 101749:44. PubMed
  220. Yao H, et al. 2020. Sci Rep. 10:17445. PubMed
  221. Ding W, et al. 2019. J Neurosci. 39:1994. PubMed
  222. Gao ML, et al. 2020. Front Cell Dev Biol. 8:128. PubMed
  223. Gao F et al. 2019. Mol Cell. 75(5):891-904 . PubMed
  224. Sanchez–Guardado L, et al. 2019. Elife. 8:e46675. PubMed
  225. Kurabayashi N, et al. 2018. Development. 145. PubMed
  226. Kawaue T, et al. 2019. Nat Commun. 10:2780. PubMed
  227. Nowakowski TJ, et al. 2018. Nat Neurosci. 21:1784. PubMed
  228. Baizabal JM et al. 2018. Neuron. 98(5):945-962 . PubMed
  229. Lin L, et al. 2020. Neuron. 107:1180. PubMed
  230. Aldinger KA, et al. 2021. Nat Neurosci. 24:1163. PubMed
  231. Carpentieri JA, et al. 2022. Nat Commun. 13:16. PubMed
  232. Meng Y, et al. 2021. Front Cell Dev Biol. 9:702462. PubMed
  233. Mathieu C, et al. 2021. MBio. 12:e0079921. PubMed
  234. C Tao, X Zhang, 2016. Cell Rep. 17:1832-1844. PubMed
  235. Damiani D, et al. 2016. Nat Commun. 7:13509. PubMed
  236. Toda T, et al. 2016. Sci Rep. 6:29578. PubMed
  237. Anda F, et al. 2016. Nat Neurosci. 10.1038/nn.4328. PubMed
  238. Martínez-Cerdeño V, et al. 2016. Cereb Cortex. 26: 374 - 383. PubMed
  239. Thomas Klein et al. 2018. Stem cell research. 31:222-226 . PubMed
  240. Han S, et al. 2018. Development. 145:. PubMed
  241. Palencia–Campos A, et al. 2017. Hum Mol Genet. 26:4556. PubMed
  242. Karzbrun E, et al. 2018. Nat Phys. 14:515. PubMed
  243. Mochizuki T, et al. 2017. Development. 144:708. PubMed
  244. Alzu'bi A et al. 2019. Journal of anatomy. 235(3):555-568 . PubMed
  245. Yan P, et al. 2020. Cell Rep. 32:107870. PubMed
  246. Campbell WA, et al. 2021. Glia. 69:1515. PubMed
  247. Li Z, et al. 2020. ACS Infect Dis. 6:2616. PubMed
  248. Too LK, et al. 2021. Retina. 41:1986. PubMed
  249. Wind M, et al. 2021. Curr Protoc. 1:e244. PubMed
  250. Conrad E, et al. 2016. Am J Physiol Endocrinol Metab. 310: E91 - E102. PubMed
  251. Wang L, Hou S, Han Y 2016. Nat Neurosci. 19: 888-896. PubMed
  252. Abdullah A, et al. 2016. Stem Cell Reports. 7:69-79. PubMed
  253. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  254. Kweon H, et al. 2021. Cell Reports. 34(8):108780. PubMed
  255. Bowles KR, et al. 2021. Cell. 184(17):4547-4563.e17. PubMed
  256. Todd L, et al. 2018. Stem Cells. 36:392. PubMed
  257. Segarra M, et al. 2018. Science. 361:eaao2861. PubMed
  258. Jin J, et al. 2019. PLoS One. 14:e0219362. PubMed
  259. Alexander JM et al. 2019. Elife. 8 pii: e41769. PubMed
  260. Zurkirchen L, et al. 2019. Nat Commun. 10:2192. PubMed
  261. Diacou R et al. 2018. Cell reports. 25(9):2510-2523 . PubMed
  262. Tang Y et al. 2018. Cell reports. 24(5):1355-1362 . PubMed
  263. Krach F, et al. 2022. Nat Commun. 13:6797. PubMed
  264. Arnst N, et al. 2021. Cells. 10:. PubMed
  265. Bockaj I, et al. 2021. PLoS Genet. 17:e1009868. PubMed
  266. Korecki AJ, et al. 2021. Gene Ther. 28:351. PubMed
  267. Besusso D, et al. 2020. Stem Cell Res. 102016:49. PubMed
  268. Jia N, et al. 2021. Stem Cell Research. 53:102367. PubMed
  269. Somatilaka BN, et al. 2020. Developmental Cell. 54(6):710-726.e8. PubMed
  270. Cugola F, et al. 2016. Nature. 534: 267-271. PubMed
  271. Radonjić N, et al. 2016. Cereb Cortex. 26: 131 - 143. PubMed
  272. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  273. Le Dréau G, et al. 2018. Elife. 7:e7267. PubMed
  274. Broix L, et al. 2018. Hum Mol Genet. 27:224. PubMed
  275. Dominguez Gonzalez B, et al. 2018. Hum Mol Genet. 27:2154. PubMed
  276. Ooi J, et al. 2019. Cell Rep. 26:2494. PubMed
  277. Hu XL, et al. 2017. Neuron. 95:309. PubMed
  278. Chang CH, et al. 2019. Dev Cell. 48:184. PubMed
  279. Chen Y, et al. 2021. Nat Methods. 18:528. PubMed
  280. Penna E, et al. 2021. Cereb Cortex Commun. 2:tgab053. PubMed
  281. Kaufman ML, et al. 2021. Development. 148:. PubMed
  282. Yao X, et al. 2022. Nat Commun. 13:3883. PubMed
  283. Yang Y, et al. 2022. Front Bioeng Biotechnol. 10:907159. PubMed
  284. Ferdaos N, et al. 2022. PLoS One. 17:e0278147. PubMed
  285. Verheyen A, et al. 2015. PLoS One. 10: 0146127. PubMed
  286. Takeo Y, et al. 2016. Sci Rep. 6: 25180. PubMed
  287. Plaisted W, et al. 2016. PLoS One. 11: 0157620. PubMed
  288. Feng J, et al. 2016. Cereb Cortex. 26: 3323 - 3334. PubMed
  289. Tsai MH, et al. 2020. Acta Neuropathol Commun. 0.406944444. PubMed
  290. Cederquist GY, et al. 2020. Cell Stem Cell. 27(1):35-49.e6. PubMed
  291. Haag D, et al. 2021. Cancer Cell. 39(3):407-422.e13. PubMed
  292. Bannier-Hlaout M, et al. 2021. Cell Stem Cell. 28(7):1221-1232.e7. PubMed
  293. Tolomeo AM, et al. 2021. Front Cell Neurosci. 602888:15. PubMed
  294. Laan L, et al. 2020. Clin Epigenetics. 12:09. PubMed
  295. Liu Z, et al. 2019. Cell Rep. 27:3832. PubMed
  296. Roy A et al. 2019. Elife. 8 pii: e45961. PubMed
  297. Cargnin F, et al. 2018. Neuron. 100:1083. PubMed
  298. Barra JM, et al. 2021. Xenotransplantation. 28:e12706. PubMed
  299. Zhang YF, et al. 2022. Front Cell Neurosci. 16:866020. PubMed
  300. Zhang XZ, et al. 2022. Front Cell Neurosci. 16:888152. PubMed
  301. Torsahakul C, et al. 2022. PLoS One. 17:e0263141. PubMed
  302. Klein JA, et al. 2022. Front Cell Neurosci. 15:794675. PubMed
  303. Patel A, et al. 2020. Development. dev193649. PubMed
  304. Žagare A, et al. 2021. STAR Protoc. 2:100524. PubMed
  305. Gao X, et al. 2021. Stem Cell Research. 53:102388. PubMed
  306. Li Z, et al. 2020. Emerg Microbes Infect. 2.044444444. PubMed
  307. Hayashi R, et al. 2017. Nat Protoc. 10.1038/nprot.2017.007. PubMed
  308. Wiley L, et al. 2016. Sci Rep. 6:30742. PubMed
RRID
AB_2749901 (BioLegend Cat. No. 901302)
AB_2565003 (BioLegend Cat. No. 901301)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
5080 View all products for this Gene ID 18508 View all products for this Gene ID 25509 View all products for this Gene ID
UniProt
View information about Pax-6 on UniProt.org

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Other Formats

View All Pax-6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Pax-6 Poly19013 WB,IHC-P,IHC-F
Go To Top Version: 9    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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