Purified anti-Pax-5 Antibody

Pricing & Availability
Clone
1H9 (See other available formats)
Other Names
Paired box 5, B-cell lineage specific activator, BSAP, paired box homeotic gene 5
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1H9_PURE_Pax5_Antibody_1_WB_073018
Western blot analysis of Daudi (lane 1) and A20 (lane 2) cells using anti-PAX-5 antibody (1H9).
  • 1H9_PURE_Pax5_Antibody_1_WB_073018
    Western blot analysis of Daudi (lane 1) and A20 (lane 2) cells using anti-PAX-5 antibody (1H9).
  • 1H9_PURE_Pax5_Antibody_2_ICC_073018
    Ramos cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-Pax-5 Antibody (clone 1H9) followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective
  • 1H9_PURE_Pax5_Antibody_3_IHC_073018
    Tissue from human tonsil sections was fixed in 10% buffered formalin overnight, processed in paraffin and placed onto SuperfrostPlus slides. Prior to staining, slides were de‐waxed by incubation at 60°C for 30 minutes followed by three 2‐minute washes in xylene. Slides were hydrated in three 2‐minute washes in absolute alcohol then washed in water for 1 minute. An antigen retrieval step followed in which slides were placed in 10mM Citric Acid + 0.05% Tween 20 (pH 6) in a microwave set on high for 30 minutes. Slides were allowed to cool to RT then washed twice (5 minutes each) in 0.05M Tris, 0.15M NaCl and 0.05% Tween 20. Endogenous peroxidase in tissue samples was blocked by applying Peroxidase Block (DakoCytomation) to the slides for 5 minutes at RT, followed by an additional wash. The slides were incubated with primary antibody (Pax5 mAb, 1:50 dilution of 0.8 mg/ml stock) for 1 hour at RT. Slides were washed and incubated with anti‐rat IgG HRP at RT for 45 minutes (1 in 50 dilution). Slides were washed and then Diaminobenzidine Plus (DakoCytomation) was applied for 5‐10 minutes. Slides were washed in distilled H2O and then counterstained in haematoxylin for 15 seconds before mounting.
Cat # Size Price Quantity Avail. Save
649702 100 µg 225 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (Pax) family of transcription factors. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Pax5 is the only member of the Pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins.  Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis.

This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse Pax-5 protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C
Application

WB - Quality tested
ICC, IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 4.0 µg per ml. For immunocytochemistryon formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The monoclonal 1H9 antibody recognizes both mouse and human Pax5.

Additional reported applications (for the relevant formats) include: Western blotting1,2 and immunohistochemical staining of formalin-fixed sections.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Kallies A, et al. 2007. Immunity 26:555. (WB)
  2. McManus S, et al. 2011. EMBO J. 30:2388. (WB)
  3. Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
RRID
AB_10683169 (BioLegend Cat. No. 649702)

Antigen Details

Structure
391 amino acids with predicted molecular weight of 42 kD
Distribution

Nucleus

Function
Plays an important role in B-cell differentiation as well as neural development and spermatogenesis; involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene
Interaction
Interacts with DAXX; binds DNA as a monomer, binds TLE4
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Liao F, et al. 1992. J. Immunol. 148:2909.
2. Nutt SL, et al. 1997. Genes Dev. 11:476.
3. Nera KP, et al. 2006. Immunity 24:283.
4. Fuxa M, et al. 2007. Immunity 178:3031.

Gene ID
5079 View all products for this Gene ID
UniProt
View information about Pax-5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 09.09.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account