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Purified anti-Nanog Antibody

Pricing & Availability
Clone
SER211 (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, ENK, ecat4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_SER211_PURE_Nanog_Antibody_WB_112818
Total cell lysates (15 µg total protein) from NIH/3T3, Raw264.7, EL4 (negative controls) and NF-1 (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of Purified anti-Nanog Antibody, clone SER211, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_SER211_PURE_Nanog_Antibody_WB_112818
    Total cell lysates (15 µg total protein) from NIH/3T3, Raw264.7, EL4 (negative controls) and NF-1 (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL of Purified anti-Nanog Antibody, clone SER211, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_SER211_PURE_Nanog_Antibody_WB_072616
    Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 cells (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Nanog antibody (clone SER211). Proteins were visualized using an HRP Goat anti-rat IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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678102 100 µg 288 CHF
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Description

Nanog is a transcription factor that plays a key role in the maintenance of embryonic stem cells pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors such as Sox2 and Oct-4. Nanog also plays a role in epithelial malignancy. Nanog is enriched in cancer stem cells and the ablation of Nanog is sufficient to reduce the cancer stem cell pool.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Nanog.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

A non-specific band of roughly 60 kD was observed in some lysates during product development testing.

RRID
AB_2565914 (BioLegend Cat. No. 678102)

Antigen Details

Structure
305 amino acids wtih a predicted molecular weight of approximately 35 kD.
Distribution

Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.

Function
Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction
Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Hwang CC, et al. 2014. PLoS One 9:e105676.
2. Torres-Padilla ME, et al. 2014. Development 141:2173.
3. Xie X, et al. 2014. Int. J. Cancer 12:2741.
4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207.
5. Kim J, et al. 2008. Cell 132:1049.
6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081.
7. Yu J, et al. 2007. Science 318:1917.

Gene ID
71950 View all products for this Gene ID
UniProt
View information about Nanog on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06.09.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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