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Purified anti-Myelin Basic Protein Phospho (Thr98) Antibody

Pricing & Availability
Clone
BL28592 (See other available formats)
Regulatory Status
RUO
Other Names
MBP, Golli-MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BL28592_PURE_MBP-Phospho-Thr98_Antibody_1_082119
Western blot of purified anti-Myelin Basic Protein Phospho (Thr98) antibody (clone BL28592). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105); Lane 4: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105; p-Thr98). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28592_PURE_MBP-Phospho-Thr98_Antibody_1_082119
    Western blot of purified anti-Myelin Basic Protein Phospho (Thr98) antibody (clone BL28592). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105); Lane 4: 5 ng of BSA-conjugated synthetic human MBP peptide (a.a 88-105; p-Thr98). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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859201 25 µg 106 CHF
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP. Phosphorylation of MBP at Thr98 has been shown to decrease its interaction with the lipid bilayer and lead to the destabilization of the compact myelin sheath.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human MBP when phosphorylated at threonine 98
Formulation
PBS, pH7.4 plus 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C
Application

WB - Quality tested
Direct ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yon M, et al. 1994. J NeuroImmunol. 58(2):121 (ELISA, WB)
RRID
AB_2814581 (BioLegend Cat. No. 859201)

Antigen Details

Structure
MBP is a 304 amino acid protein with predicted molecular mass of ~33 kD. MPB exists in multiple isoforms with observed molecular masses of ~ 18, 21 and 33 kD.
Distribution

Tissue Distribution: Central and peripheral nervous system
Cellular Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Interaction
MBP forms homodimer
Cell Type
Oligodendrocytes
Biology Area
Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Llyasov AA, et al. 2018. Front Neurosci. 12:733
  2. Muller C, et al. 2013. Front Cell Neurosci. 7:169
  3. Harauz G, et al. 2013. J Neurochem. 125(3):334
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein Phospho Thr98 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08.21.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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