Loading...

Purified anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
H57-597_Pure_071608.jpg
C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_Pure_071608.jpg
    C57L/6 splenocytes stained with H57-597 purified, followed by anti-Armenian hamster IgG FITC
  • H57-597_pure_TCR-Beta_Antibody_IHC-F_102518
    Fresh, frozen mouse spleen was stained with purified TCR β chain clone H57-597 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Compare all formats
Cat # Size Price Quantity Check Availability Save
109201 50 µg 54 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109202 500 µg 138 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F  - Verified
IP, Costim, Depletion - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Tsukumo S, et al. 2006. J Immunol. 177:8365. PubMed
  3. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  4. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  5. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  6. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  7. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  8. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  9. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  10. McDole J, et al. 2010. Am J Pathol. 177:1823. PubMed
  11. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  12. Tan A, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.004. PubMed
  13. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  14. Willimsky G, et al. 2021. Elife. 10:. PubMed
  15. Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
  16. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  17. Lee JH, et al. 2022. iScience. 25:104166. PubMed
  18. Peng V, et al. 2020. J Biol Chem. 295:14866. PubMed
  19. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  20. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  21. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  22. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  23. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  24. Sharba S, et al. 2019. Virulence. 10:97. PubMed
  25. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  26. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  27. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  28. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  29. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  30. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  31. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  32. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  33. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  34. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  35. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  36. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  37. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  38. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  39. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  40. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  41. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  42. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  43. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  44. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  45. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  46. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  47. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  48. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  49. Sugiyarto G, et al. 2021. Immunother Adv. 1:ltaa001. PubMed
  50. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  51. Dubik M, et al. 2021. Front Neurosci. 15:682451. PubMed
  52. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
RRID
AB_313424 (BioLegend Cat. No. 109201)
AB_313425 (BioLegend Cat. No. 109202)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 5    Revision Date: 08.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account