Purified anti-mouse IL-22 Antibody

Pricing & Availability
Clone
Poly5164 (See other available formats)
Other Names
Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF
Isotype
Goat Polyclonal IgG
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516401 100 µg 225 CHF
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Description

IL-22 is a cytokine structurally related to IL-10.  Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD.  It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.  It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells.  AhR activation increases Th17 polarization and induces IL-22 production.  IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ.  IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin.  IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease.  Moreover, it plays a critical role in mucosal immunity and the wound healing process.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2 and 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 250-3.9 pg/ml are recommended.

Application Notes

ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard.

In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively.

Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations.

Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended.

Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1

Application References

(PubMed link indicates BioLegend citation)
  1. Veldhoen M, et al. 2009. J. Exp. Med. 206:43.
RRID
AB_2561332 (BioLegend Cat. No. 516401)

Antigen Details

Structure
A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Distribution
Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.
Function
Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing.
Cell Type
Th17, Th1, Th2, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Martin B, et al. 2009. Immunity 31:321.
2. Liang SC, et al. 2006. J. Exp. Med. 203:2271.
3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43.
4. Zheng Y, et al. 2007. Nature 445:648.
5. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
6. Wolk K, et al. 2007. J. Immunol. 178:5973.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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